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Team:ETH Zurich/Achievements/Data page
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<!--[[File:ETHZ Data page.png|850px|center|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]]--> | <!--[[File:ETHZ Data page.png|850px|center|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]]--> | ||
Revision as of 17:44, 25 October 2011
Contents |
Data page
SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
How Our System Works
Data For Our Favorite New Parts
- [http://partsregistry.org/Part:BBa_K625000 MainPage]-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
- [http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. (Characterization)
- [http://partsregistry.org/Part:BBa_K625003 MainPage]-PU promoter, BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization)
Characterized Existing Parts
- [http://partsregistry.org/Part:BBa_K625001 MainPage]- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
- [http://partsregistry.org/Part:BBa_K625002 MainPage]-PU promoter, BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)
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