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Team:ETH Zurich/Process/Validation
From 2011.igem.org
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We checked whether our designed works by putting an engineered cells that produce GFP upon arabinose induction in agarose and filling the channel with it. In the reservoir we put arabinose in ____________ ?? After _____?? we optained a nice arabinose-inducible GFP gradient. | We checked whether our designed works by putting an engineered cells that produce GFP upon arabinose induction in agarose and filling the channel with it. In the reservoir we put arabinose in ____________ ?? After _____?? we optained a nice arabinose-inducible GFP gradient. | ||
| + | [[File:ETHZ_arabinose_gradient.png|500px|center|thumb|'''Figure 1: Proof of principle, arabinose-inducible GFP gradient created in the microfluidic devise ''' ]] | ||
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Revision as of 14:40, 23 October 2011
| Final Setup and Validation |
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| This page presents description of our final channel design as well as description of its construction, which we did by ourselves. Our final channel is build out of PDMS and constructed with a technique called photolithography. We are also presenting the experiments we did to validate our setup and to show that it can work in practice | ||||
Final channel constructionTo construct our final channel, we used the technique photolithography. Photolithography is the photopatterning of channels from a mask (drawing of channels in 2D) and is based on the utilization of particular substances (photoresists) that become soluble to particular solvents after being exposed to UV light [1].
@BIOLOGISTS: I need some information about how exactly you constructed the channel. can you ask Olivier maybe to give you some proticols? I can then write them down. Can you also take a look at the reference? Is this what you did? Thanks You can see below some photos of the channels and the channel building process:
References: [1] http://www.elveflow.com/microfluidic/16-start-with-microfluidics |
Setup Validation@BIOLOGISTS: Please write here, cuz I don't know what you did exactly :SWe checked whether our designed works by putting an engineered cells that produce GFP upon arabinose induction in agarose and filling the channel with it. In the reservoir we put arabinose in ____________ ?? After _____?? we optained a nice arabinose-inducible GFP gradient.  |
