Team:Osaka/week4

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{{Osaka}}
{{Osaka}}
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__NOTOC__
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==August 21 (Sun)==
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==August 21(Sun)==
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# Preparation of LB agar plates (14Tet)
# Preparation of LB agar plates (14Tet)
# Transfer to liquid culture:01,02,03,04
# Transfer to liquid culture:01,02,03,04
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==August 22(Mon)==
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==August 22 (Mon)==
# Colony check
# Colony check
#* Transformed cells produced colonies!
#* Transformed cells produced colonies!
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# Transfer to liquid culture:1-7A
# Transfer to liquid culture:1-7A
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==August 23(Tue)==
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==August 23 (Tue)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
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#* 1-7A  ok
#* 1-7A  ok
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==August 24(Wed)==
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==August 24 (Wed)==
# Gel electrophoresis of 1-7A
# Gel electrophoresis of 1-7A
# Gel extraction of 1-7A
# Gel extraction of 1-7A
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==August 25(Thu)==
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==August 25 (Thu)==
# Transfer to liquid culture:08,4-15M
# Transfer to liquid culture:08,4-15M
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==August 26(Fri)==
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==August 26 (Fri)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
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# Transfer to liquid culture:08
# Transfer to liquid culture:08
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==August 27(Sat)==
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==August 27 (Sat)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
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[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]
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week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|'''4'''|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]
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week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|'''4'''|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]

Latest revision as of 05:53, 19 October 2011

August 21 (Sun)

  1. Preparation of LB agar plates (14Tet)
  2. Transfer to liquid culture:01,02,03,04
  3. Transformaton of Registry parts (See Table 4).
Table4
IDPart NameResistanceDescription
1-7A<bbpart>BBa_J04450</bbpart>T construction plasmid containing mRFP coding device

August 22 (Mon)

  1. Colony check
    • Transformed cells produced colonies!
    • Non-transformed cells (negative controls) did not grow on Tet plates -> confirmed lack of natural antibiotic resistance
  2. Transfer to liquid culture:1-7A

August 23 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 1-7A ok

August 24 (Wed)

  1. Gel electrophoresis of 1-7A
  2. Gel extraction of 1-7A
  3. Ligation
    • 08(T):07(upstream)+1-23L(downstream)+1-7A(vector)
  4. Transformation of 08 and Registry parts (see Table5)
Table5
IDPart NameResistanceDescription
4-15M<bbpart>BBa_K325101</bbpart>C EPIC Firefly Luciferase Photinus Pyralis (E. coli optimised)

August 25 (Thu)

  1. Transfer to liquid culture:08,4-15M


August 26 (Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 08 ×
    • 4-15M ok
  4. Ligation
    • 09(K):1-9N(upstream)+4-15M(downstream)+1-5A(vector)
    • 10(K):1-1D(upstream)+4-15M(downstream)+1-5A(vector)
  5. Transfer to liquid culture:08

August 27 (Sat)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 08 ok
  4. Ligation
    • 11(A):08(upstream)+06(downstream)+1-1G(vector)
  5. Transformation of 09,10,11
  6. Transfer to liquid culture:04


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