Team:Osaka/week6

From 2011.igem.org

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{{Osaka}}
{{Osaka}}
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__NOTOC__
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==September 4 (Sun)==
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# Restriction digests:3-10C,1-2M
 +
# Gel extraction of digests
 +
# Ligation
 +
#* 03:1-9N+3-6H  again
 +
# Transformation of ligation products
 +
# Photospectrometry measuament
 +
==September 5 (Mon)==
 +
# Absorbance measurement
 +
# To liquid culture:03,17,3-10C
-
==September 4(Sun)==
 
 +
==September 6 (Tue)==
 +
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
#* all parts ×
 +
# PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
 +
# Gel Electrophoresis of PCR products
 +
#* PprM ok
 +
#* PprI,PprA,RecA  ×
 +
# PCR
 +
# Gel Electrophoresis of PCR products
 +
#* ×
-
==September 5(Mon)==
 
-
==September 6(Tue)==
+
==September 7 (Wed)==
 +
# PCR
 +
# Gel electrophoresis of PCR products
 +
#* PprM ok
 +
#* PprI,PprA,RecA  ×
 +
# Gel electrophoresis of PprM
 +
# Gel extraction
 +
# PCR
 +
# Gel electrophoresis of PCR products
 +
#* PprI,PprA,RecA  ×
 +
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Gel electrophoresis of digests
 +
#* 03 ok
 +
# To liquid culture:03,04 (preincubation)
 +
# PCR
 +
# Electrophoresis of PCR products
 +
# PCR
 +
==September 8 (Thu)==
 +
# Electrophoresis of PCR products
 +
# Restriction digests of 1-3A(backbone)
 +
# PCR
 +
# Electrophoresis of PCR products and miniprepped parts
 +
# Gel extraction of 1-3A
 +
# PCR
 +
# Electrophoresis of PCR products
 +
# Inoculation of 03,04
 +
# To liquid culture 03,04
 +
# PCR
 +
# Electrophoresis of PCR products
-
==September 7(Wed)==
 
-
==September 8(Thu)==
+
==September 9 (Fri)==
 +
# PCR
 +
# Electrophoresis of PCR products
 +
# Gel extraction
 +
# Restriction digests
 +
# PCR
 +
# Electrophoresis of PCR products
 +
# Gel extraction
 +
# Restriction digests
 +
# Ligation
 +
#* 101(C):PprI+1-3A(vector)
 +
#* 102(C):PprA+1-3A(vector)
 +
# Transformation of ligation products
 +
# Absorbance measurement
 +
==September 10 (Sat)==
 +
# PCR
 +
# Electrophoresis of PCR products
 +
# To liquid culture:101,102
 +
# PCR
 +
# PCR purification
 +
# Absorbance measurement
-
==September 9(Fri)==
 
-
==September 10(Sat)==
+
[[Team:Osaka/Notebook|Back to Notebook]]
-
 
+
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|'''6'''|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]
-
 
+
-
[[Team:Osaka/Notebook|Back to Notebook]]
+

Latest revision as of 05:51, 19 October 2011

September 4 (Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5 (Mon)

  1. Absorbance measurement
  2. To liquid culture:03,17,3-10C


September 6 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • all parts ×
  4. PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
  5. Gel Electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  6. PCR
  7. Gel Electrophoresis of PCR products
    • ×


September 7 (Wed)

  1. PCR
  2. Gel electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  3. Gel electrophoresis of PprM
  4. Gel extraction
  5. PCR
  6. Gel electrophoresis of PCR products
    • PprI,PprA,RecA ×
  7. Miniprep of yesterday's culture
  8. Restriction digests of miniprepped parts
  9. Gel electrophoresis of digests
    • 03 ok
  10. To liquid culture:03,04 (preincubation)
  11. PCR
  12. Electrophoresis of PCR products
  13. PCR

September 8 (Thu)

  1. Electrophoresis of PCR products
  2. Restriction digests of 1-3A(backbone)
  3. PCR
  4. Electrophoresis of PCR products and miniprepped parts
  5. Gel extraction of 1-3A
  6. PCR
  7. Electrophoresis of PCR products
  8. Inoculation of 03,04
  9. To liquid culture 03,04
  10. PCR
  11. Electrophoresis of PCR products


September 9 (Fri)

  1. PCR
  2. Electrophoresis of PCR products
  3. Gel extraction
  4. Restriction digests
  5. PCR
  6. Electrophoresis of PCR products
  7. Gel extraction
  8. Restriction digests
  9. Ligation
    • 101(C):PprI+1-3A(vector)
    • 102(C):PprA+1-3A(vector)
  10. Transformation of ligation products
  11. Absorbance measurement

September 10 (Sat)

  1. PCR
  2. Electrophoresis of PCR products
  3. To liquid culture:101,102
  4. PCR
  5. PCR purification
  6. Absorbance measurement


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

Retrieved from "http://2011.igem.org/Team:Osaka/week6"