Team:Osaka/week6

From 2011.igem.org

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week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|'''6'''|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]
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week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|'''6'''|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]

Latest revision as of 05:51, 19 October 2011

September 4 (Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5 (Mon)

  1. Absorbance measurement
  2. To liquid culture:03,17,3-10C


September 6 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • all parts ×
  4. PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
  5. Gel Electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  6. PCR
  7. Gel Electrophoresis of PCR products
    • ×


September 7 (Wed)

  1. PCR
  2. Gel electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  3. Gel electrophoresis of PprM
  4. Gel extraction
  5. PCR
  6. Gel electrophoresis of PCR products
    • PprI,PprA,RecA ×
  7. Miniprep of yesterday's culture
  8. Restriction digests of miniprepped parts
  9. Gel electrophoresis of digests
    • 03 ok
  10. To liquid culture:03,04 (preincubation)
  11. PCR
  12. Electrophoresis of PCR products
  13. PCR

September 8 (Thu)

  1. Electrophoresis of PCR products
  2. Restriction digests of 1-3A(backbone)
  3. PCR
  4. Electrophoresis of PCR products and miniprepped parts
  5. Gel extraction of 1-3A
  6. PCR
  7. Electrophoresis of PCR products
  8. Inoculation of 03,04
  9. To liquid culture 03,04
  10. PCR
  11. Electrophoresis of PCR products


September 9 (Fri)

  1. PCR
  2. Electrophoresis of PCR products
  3. Gel extraction
  4. Restriction digests
  5. PCR
  6. Electrophoresis of PCR products
  7. Gel extraction
  8. Restriction digests
  9. Ligation
    • 101(C):PprI+1-3A(vector)
    • 102(C):PprA+1-3A(vector)
  10. Transformation of ligation products
  11. Absorbance measurement

September 10 (Sat)

  1. PCR
  2. Electrophoresis of PCR products
  3. To liquid culture:101,102
  4. PCR
  5. PCR purification
  6. Absorbance measurement


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