Team:Osaka/week6

From 2011.igem.org

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{{Osaka}}
{{Osaka}}
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__NOTOC__
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==September 4 (Sun)==
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==September 4(Sun)==
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# Restriction digests:3-10C,1-2M
# Restriction digests:3-10C,1-2M
# Gel extraction of digests
# Gel extraction of digests
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# Photospectrometry measuament
# Photospectrometry measuament
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==September 5(Mon)==
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==September 5 (Mon)==
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
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# Absorbance measurement
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|+Table 1
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# To liquid culture:03,17,3-10C
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!Name!!IPTG(0.1M)!!incubation!!OD600!!A474
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|-
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|1-9N||<bbpart>BBa_J22106</bbpart>||A|| rec A (SOS) Promoter
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-
|-
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|1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS
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-
|-
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-
|1-14K||<bbpart>BBa_B0040</bbpart>||A||green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP
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|-
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|1-12M||<bbpart>BBa_E0240</bbpart>||A||GFP generator
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-
|-
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|1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd)
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-
|}
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==September 6(Tue)==
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==September 6 (Tue)==
 +
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
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# Gel electrophoresis of digests
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#* all parts ×
 +
# PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
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# Gel Electrophoresis of PCR products
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#* PprM ok
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#* PprI,PprA,RecA  ×
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# PCR
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# Gel Electrophoresis of PCR products
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#* ×
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==September 7(Wed)==
 
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==September 7 (Wed)==
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# PCR
 +
# Gel electrophoresis of PCR products
 +
#* PprM ok
 +
#* PprI,PprA,RecA  ×
 +
# Gel electrophoresis of PprM
 +
# Gel extraction
 +
# PCR
 +
# Gel electrophoresis of PCR products
 +
#* PprI,PprA,RecA  ×
 +
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Gel electrophoresis of digests
 +
#* 03 ok
 +
# To liquid culture:03,04 (preincubation)
 +
# PCR
 +
# Electrophoresis of PCR products
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# PCR
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==September 8(Thu)==
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==September 8 (Thu)==
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# Electrophoresis of PCR products
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# Restriction digests of 1-3A(backbone)
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# PCR
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# Electrophoresis of PCR products and miniprepped parts
 +
# Gel extraction of 1-3A
 +
# PCR
 +
# Electrophoresis of PCR products
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# Inoculation of 03,04
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# To liquid culture 03,04
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# PCR
 +
# Electrophoresis of PCR products
-
==September 9(Fri)==
 
 +
==September 9 (Fri)==
 +
# PCR
 +
# Electrophoresis of PCR products
 +
# Gel extraction
 +
# Restriction digests
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# PCR
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# Electrophoresis of PCR products
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# Gel extraction
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# Restriction digests
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# Ligation
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#* 101(C):PprI+1-3A(vector)
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#* 102(C):PprA+1-3A(vector)
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# Transformation of ligation products
 +
# Absorbance measurement
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==September 10(Sat)==
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==September 10 (Sat)==
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# PCR
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# Electrophoresis of PCR products
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# To liquid culture:101,102
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# PCR
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# PCR purification
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# Absorbance measurement
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]
 +
 +
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|'''6'''|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]

Latest revision as of 05:51, 19 October 2011

September 4 (Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5 (Mon)

  1. Absorbance measurement
  2. To liquid culture:03,17,3-10C


September 6 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • all parts ×
  4. PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
  5. Gel Electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  6. PCR
  7. Gel Electrophoresis of PCR products
    • ×


September 7 (Wed)

  1. PCR
  2. Gel electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  3. Gel electrophoresis of PprM
  4. Gel extraction
  5. PCR
  6. Gel electrophoresis of PCR products
    • PprI,PprA,RecA ×
  7. Miniprep of yesterday's culture
  8. Restriction digests of miniprepped parts
  9. Gel electrophoresis of digests
    • 03 ok
  10. To liquid culture:03,04 (preincubation)
  11. PCR
  12. Electrophoresis of PCR products
  13. PCR

September 8 (Thu)

  1. Electrophoresis of PCR products
  2. Restriction digests of 1-3A(backbone)
  3. PCR
  4. Electrophoresis of PCR products and miniprepped parts
  5. Gel extraction of 1-3A
  6. PCR
  7. Electrophoresis of PCR products
  8. Inoculation of 03,04
  9. To liquid culture 03,04
  10. PCR
  11. Electrophoresis of PCR products


September 9 (Fri)

  1. PCR
  2. Electrophoresis of PCR products
  3. Gel extraction
  4. Restriction digests
  5. PCR
  6. Electrophoresis of PCR products
  7. Gel extraction
  8. Restriction digests
  9. Ligation
    • 101(C):PprI+1-3A(vector)
    • 102(C):PprA+1-3A(vector)
  10. Transformation of ligation products
  11. Absorbance measurement

September 10 (Sat)

  1. PCR
  2. Electrophoresis of PCR products
  3. To liquid culture:101,102
  4. PCR
  5. PCR purification
  6. Absorbance measurement


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

Retrieved from "http://2011.igem.org/Team:Osaka/week6"