Team:Osaka/week8
From 2011.igem.org
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- | + | ==September 18 (Sun)== | |
- | ==September 18(Sun)== | + | |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
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# To liquid culture:03,105,106,107,108,109,110,111,112 | # To liquid culture:03,105,106,107,108,109,110,111,112 | ||
- | ==September 19(Mon)== | + | ==September 19 (Mon)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
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# Transformation of ligation products | # Transformation of ligation products | ||
# Plating (cell survival) | # Plating (cell survival) | ||
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- | ==September 20(Tue)== | + | ==September 20 (Tue)== |
# To liquid culture:113,114 | # To liquid culture:113,114 | ||
# colony check (cell survival) | # colony check (cell survival) | ||
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- | ==September 21(Wed)== | + | ==September 21 (Wed)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
Line 231: | Line 35: | ||
- | ==September 22(Thu)== | + | ==September 22 (Thu)== |
# Colony check | # Colony check | ||
# Plating | # Plating | ||
# To liquid culture | # To liquid culture | ||
- | ==September 23(Fri)== | + | ==September 23 (Fri)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
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# Colony check | # Colony check | ||
- | ==September 24(Sat)== | + | ==September 24 (Sat)== |
- | + | # Preparation of LB plates | |
+ | # Plating | ||
[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] | ||
- | week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|'''8'''|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13 | + | week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|'''8'''|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]] |
Latest revision as of 05:51, 19 October 2011
September 18 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:03,105,106,107,108,109,110,111,112
September 19 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 113(T):105(upstream)+110(downstream)+1-7A(vector)
- 114(T):111(upstream)+112(downstream)+1-7A(vector)
- Transformation of ligation products
- Plating (cell survival)
September 20 (Tue)
- To liquid culture:113,114
- colony check (cell survival)
September 21 (Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 115(A):113(upstream)+114(downstream)+1-1G(vector)
- Transformation of ligation products
- colony check (cell survival)
- Plating (cell survival)
September 22 (Thu)
- Colony check
- Plating
- To liquid culture
September 23 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Colony check
September 24 (Sat)
- Preparation of LB plates
- Plating
week 1|2|3|4|5|6|7|8|9|10|11|12|13