Team:Osaka/week8

From 2011.igem.org

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{{Osaka}}
{{Osaka}}
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__NOTOC__
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==September 18 (Sun)==
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==September 18(Sun)==
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# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
Line 8: Line 7:
# To liquid culture:03,105,106,107,108,109,110,111,112
# To liquid culture:03,105,106,107,108,109,110,111,112
-
==September 19(Mon)==
+
==September 19 (Mon)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
Line 18: Line 17:
# Transformation of ligation products
# Transformation of ligation products
# Plating (cell survival)
# Plating (cell survival)
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
-
|+Table 10
 
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!Name!!OD600!!UV(mJ/cm^2)
 
-
|-
 
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|PprI-1||0.0109||0
 
-
|-
 
-
|PprI-2||0.0109||0.5
 
-
|-
 
-
|PprI-3||0.0109||1.0
 
-
|-
 
-
|PprI-4||0.0109||2.0
 
-
|-
 
-
|PprI-5||0.0109||5.0
 
-
|-
 
-
|PprA-1||0.0418||0
 
-
|-
 
-
|PprA-2||0.0418||0.5
 
-
|-
 
-
|PprA-3||0.0418||1.0
 
-
|-
 
-
|PprA-4||0.0418||2.0
 
-
|-
 
-
|PprA-5||0.0418||5.0
 
-
|-
 
-
|PprM-1||0.0454||0
 
-
|-
 
-
|PprM-2||0.0454||0.5
 
-
|-
 
-
|PprM-3||0.0454||1.0
 
-
|-
 
-
|PprM-4||0.0454||2.0
 
-
|-
 
-
|PprM-5||0.0454||5.0
 
-
|-
 
-
|RecA-1||0.0114||0
 
-
|-
 
-
|RecA-2||0.0114||0.5
 
-
|-
 
-
|RecA-3||0.0114||1.0
 
-
|-
 
-
|RecA-4||0.0114||2.0
 
-
|-
 
-
|RecA-5||0.0114||5.0
 
-
|-
 
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|empty vector-1||0.0515||0
 
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|-
 
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|empty vector-2||0.0515||0.5
 
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|-
 
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|empty vector-3||0.0515||1.0
 
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|-
 
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|empty vector-4||0.0515||2.0
 
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|-
 
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|empty vector-5||0.0515||5.0
 
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|-
 
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|wild type-1||0.0546||0
 
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|-
 
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|wild type-2||0.0546||0.5
 
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|-
 
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|wild type-3||0.0546||1.0
 
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|-
 
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|wild type-4||0.0546||2.0
 
-
|-
 
-
|wild type-5||0.0546||5.0
 
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|}
 
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==September 20(Tue)==
+
==September 20 (Tue)==
# To liquid culture:113,114
# To liquid culture:113,114
# colony check (cell survival)
# colony check (cell survival)
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
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|+Table 11
 
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!Name!!UV(mJ/cm^2)!!conoly counts
 
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|-
 
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|PprI-1||0||
 
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|-
 
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|PprI-2||0.5||
 
-
|-
 
-
|PprI-3||1.0||
 
-
|-
 
-
|PprI-4||2.0||
 
-
|-
 
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|PprI-5||5.0||
 
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|-
 
-
|PprA-1||0||
 
-
|-
 
-
|PprA-2||0.5||
 
-
|-
 
-
|PprA-3||1.0||
 
-
|-
 
-
|PprA-4||2.0||
 
-
|-
 
-
|PprA-5||5.0||
 
-
|-
 
-
|PprM-1||0||
 
-
|-
 
-
|PprM-2||0.5||
 
-
|-
 
-
|PprM-3||1.0||
 
-
|-
 
-
|PprM-4||2.0||
 
-
|-
 
-
|PprM-5||5.0||
 
-
|-
 
-
|RecA-1||0||
 
-
|-
 
-
|RecA-2||0.5||
 
-
|-
 
-
|RecA-3||1.0||
 
-
|-
 
-
|RecA-4||2.0||
 
-
|-
 
-
|RecA-5||5.0||
 
-
|-
 
-
|Empty vector-1||0||
 
-
|-
 
-
|Empty vector-2||0.5||
 
-
|-
 
-
|Empty vector-3||1.0||
 
-
|-
 
-
|Empty vector-4||2.0||
 
-
|-
 
-
|Empty vector-5||5.0||
 
-
|-
 
-
|wild type-1||0||
 
-
|-
 
-
|wild type-2||0.5||
 
-
|-
 
-
|wild type-3||1.0||
 
-
|-
 
-
|wild type-4||2.0||
 
-
|-
 
-
|wild type-5||5.0||
 
-
|}
 
-
# Plating (cell survival)
 
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
-
|+Table 12
 
-
!Name!!OD600!!UV(mJ/cm^2)
 
-
|-
 
-
|PprI-1||0.0109||0
 
-
|-
 
-
|PprI-2||0.0109||1.0
 
-
|-
 
-
|PprI-3||0.0109||2.0
 
-
|-
 
-
|PprI-4||0.0109||3.0
 
-
|-
 
-
|PprI-5||0.0109||4.0
 
-
|-
 
-
|PprA-1||0.0418||0
 
-
|-
 
-
|PprA-2||0.0418||1.0
 
-
|-
 
-
|PprA-3||0.0418||2.0
 
-
|-
 
-
|PprA-4||0.0418||3.0
 
-
|-
 
-
|PprA-5||0.0418||4.0
 
-
|-
 
-
|PprM-1||0.0454||0
 
-
|-
 
-
|PprM-2||0.0454||1.0
 
-
|-
 
-
|PprM-3||0.0454||2.0
 
-
|-
 
-
|PprM-4||0.0454||3.0
 
-
|-
 
-
|PprM-5||0.0454||4.0
 
-
|-
 
-
|RecA-1||0.0114||0
 
-
|-
 
-
|RecA-2||0.0114||1.0
 
-
|-
 
-
|RecA-3||0.0114||2.0
 
-
|-
 
-
|RecA-4||0.0114||3.0
 
-
|-
 
-
|RecA-5||0.0114||4.0
 
-
|-
 
-
|empty vector-1||0.0515||0
 
-
|-
 
-
|empty vector-2||0.0515||1.0
 
-
|-
 
-
|empty vector-3||0.0515||2.0
 
-
|-
 
-
|empty vector-4||0.0515||3.0
 
-
|-
 
-
|empty vector-5||0.0515||4.0
 
-
|-
 
-
|wild type-1||0.0546||0
 
-
|-
 
-
|wild type-2||0.0546||1.0
 
-
|-
 
-
|wild type-3||0.0546||2.0
 
-
|-
 
-
|wild type-4||0.0546||3.0
 
-
|-
 
-
|wild type-5||0.0546||4.0
 
-
|}
 
-
 
-
 
-
==September 21(Wed)==
+
==September 21 (Wed)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
Line 231: Line 35:
-
==September 22(Thu)==
+
==September 22 (Thu)==
 +
# Colony check
 +
# Plating
 +
# To liquid culture
 +
==September 23 (Fri)==
 +
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
#* ok
 +
# Colony check
-
==September 23(Fri)==
+
==September 24 (Sat)==
-
 
+
# Preparation of LB plates
-
 
+
# Plating
-
==September 24(Sat)==
+
-
 
+
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]
-
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|'''8'''|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]|[[Team:Osaka/week14|14]]
+
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|'''8'''|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]

Latest revision as of 05:51, 19 October 2011

September 18 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Plating (cell survival)


September 20 (Tue)

  1. To liquid culture:113,114
  2. colony check (cell survival)

September 21 (Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 115(A):113(upstream)+114(downstream)+1-1G(vector)
  5. Transformation of ligation products
  6. colony check (cell survival)
  7. Plating (cell survival)


September 22 (Thu)

  1. Colony check
  2. Plating
  3. To liquid culture

September 23 (Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Colony check

September 24 (Sat)

  1. Preparation of LB plates
  2. Plating


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

Retrieved from "http://2011.igem.org/Team:Osaka/week8"