Team:Osaka/week8

From 2011.igem.org

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{{Osaka}}
{{Osaka}}
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__NOTOC__
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==September 18 (Sun)==
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==September 18(Sun)==
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# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
Line 8: Line 7:
# To liquid culture:03,105,106,107,108,109,110,111,112
# To liquid culture:03,105,106,107,108,109,110,111,112
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==September 19(Mon)==
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==September 19 (Mon)==
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
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#* 114(T):111(upstream)+112(downstream)+1-7A(vector)
#* 114(T):111(upstream)+112(downstream)+1-7A(vector)
# Transformation of ligation products
# Transformation of ligation products
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# Measurment of cell survival
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# Plating (cell survival)
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
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|+Table 7
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!Name!!OD600!!UV(mJ/cm^2)
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|-
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|PprI||0.0109||0,0.5,1,2,5
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|-
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|PprA||0.0418||0,0.5,1,2,5
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|-
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|PprM||0.0454||0,0.5,1,2,5
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|-
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|RecA||0.0114||0,0.5,1,2,5
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|-
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|empty vector||0.0515||0,0.5,1,2,5
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|-
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|wild type||0.0546||0,0.5,1,2,5
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|}
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==September 20(Tue)==
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==September 20 (Tue)==
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# To liquid culture:113,114
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# colony check (cell survival)
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==September 21 (Wed)==
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# Miniprep of yesterday's culture
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# Restriction digests of minipreppped parts
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# Gel electrophoresis of digests
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#* ok
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# Ligation
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#* 115(A):113(upstream)+114(downstream)+1-1G(vector)
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# Transformation of ligation products
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# colony check (cell survival)
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# Plating (cell survival)
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==September 21(Wed)==
 
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==September 22 (Thu)==
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# Colony check
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# Plating
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# To liquid culture
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==September 22(Thu)==
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==September 23 (Fri)==
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# Miniprep of yesterday's culture
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# Restriction digests of minipreppped parts
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# Gel electrophoresis of digests
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#* ok
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# Colony check
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==September 24 (Sat)==
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# Preparation of LB plates
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# Plating
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==September 23(Fri)==
 
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==September 24(Sat)==
 
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[[Team:Osaka/Notebook|Back to Notebook]]
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week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|'''8'''|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]
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[[Team:Osaka/Notebook|Back to Notebook]]
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Latest revision as of 05:51, 19 October 2011

September 18 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Plating (cell survival)


September 20 (Tue)

  1. To liquid culture:113,114
  2. colony check (cell survival)

September 21 (Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 115(A):113(upstream)+114(downstream)+1-1G(vector)
  5. Transformation of ligation products
  6. colony check (cell survival)
  7. Plating (cell survival)


September 22 (Thu)

  1. Colony check
  2. Plating
  3. To liquid culture

September 23 (Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Colony check

September 24 (Sat)

  1. Preparation of LB plates
  2. Plating


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

Retrieved from "http://2011.igem.org/Team:Osaka/week8"