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Team:Tokyo Metropolitan/Notebook/S14
From 2011.igem.org
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| + | @colony PCR oriTr-GFP-oriTr | ||
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| + | @electrophoresis pSB1C3 | ||
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| + | @preculturefrom master plate of colony PCR | ||
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| + | = -Targeting- = | ||
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| + | Preculture | ||
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| + | P0440（2010） | ||
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| + | Colony PCR | ||
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| + | Colony PCR J23100-K317037-K283030. | ||
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| + | Electrophoresis | ||
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| + | Electrophoresis colony PCR J23100-K317037-K283030 and find band only No.32. | ||
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| + | Preculture | ||
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| + | J23100-K317037-K283030 No.32 | ||
Latest revision as of 14:06, 5 October 2011
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-Visualization-
-Electrophoresis
Digested samples since one day ago
-Ligation
Promoter - suppressed expression system
-Transformation
Above samples
@colony PCR oriTr-GFP-oriTr
@electrophoresis pSB1C3
@preculturefrom master plate of colony PCR
-Targeting-
Preculture
P0440（2010）
Colony PCR
Colony PCR J23100-K317037-K283030.
Electrophoresis
Electrophoresis colony PCR J23100-K317037-K283030 and find band only No.32.
Preculture
J23100-K317037-K283030 No.32
