"
Team:Tokyo Metropolitan/Notebook/S09
From 2011.igem.org
(Created page with "{{:Team:Tokyo_Metropolitan/Header}} <html> <div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;"> </html> -Plasmid extraction and DNA refining Ex...") |
|||
| (2 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
<div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;"> | <div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;"> | ||
</html> | </html> | ||
| + | |||
| + | = -Targeting- = | ||
-Plasmid extraction and DNA refining | -Plasmid extraction and DNA refining | ||
| Line 46: | Line 48: | ||
Setrilised for nine tubes 4 ml. | Setrilised for nine tubes 4 ml. | ||
| + | |||
| + | |||
| + | = -Visualization- = | ||
| + | -Plasmid extraction | ||
| + | |||
| + | Promoter | ||
| + | |||
| + | Suppressed expression system | ||
| + | |||
| + | RFP | ||
| + | |||
| + | |||
| + | -DNA refinig | ||
| + | |||
| + | Above samples | ||
| + | |||
| + | |||
| + | ------ | ||
| + | |||
| + | |||
| + | |||
| + | @plasmid extraction, DNA refine, DNA densitometry, restrictin emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP-OriTr | ||
| + | |||
| + | @excess preculture medium move to media(Amp). | ||
Latest revision as of 14:01, 5 October 2011
 |
 |
 |
 |
 |
 |
 |
 |
-Targeting-
-Plasmid extraction and DNA refining
Extracted plasmid and refined DNA used Cell Biochemical Lab's kits for No. 29 of J23100-K317037 and No. 19 of J23100-P0440.
Results measured concentration
No. 29 of J23100-K317037 was 150.
No. 19 of J23100-P0440 was 138.
J22005
I. 105
II. 155
III. 62
concentration was ng/ul
-Treat restriction enzyme
J23100-K317037 -> S and P
J23100-P0440 -> E and P
K283030 -> X and P
J22005 -> N
pSB1C3 -> E and P
incubated at 37 deg C after treat with the preceding enzymes.
-Setrilization for liquid medium
Setrilised for nine tubes 4 ml.
-Visualization-
-Plasmid extraction
Promoter
Suppressed expression system
RFP
-DNA refinig
Above samples
@plasmid extraction, DNA refine, DNA densitometry, restrictin emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP-OriTr
@excess preculture medium move to media(Amp).
