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Team:Tokyo Metropolitan/Notebook/S05
From 2011.igem.org
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| - | Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell. | + | '''-Molecular Genetics Microbiology-''' |
| - | + | Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. | |
| + | Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell. | ||
Amplified and purification I20260 with PCR. | Amplified and purification I20260 with PCR. | ||
| - | |||
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI | Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI | ||
Revision as of 04:47, 5 October 2011
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-Molecular Genetics Microbiology- Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell. Amplified and purification I20260 with PCR. Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
