Team:Osaka/week7
From 2011.igem.org
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==September 11 (Sun)== | ==September 11 (Sun)== | ||
# Gel extraction of digests | # Gel extraction of digests |
Revision as of 11:41, 4 October 2011
September 11 (Sun)
- Gel extraction of digests
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 103(C):PprM+1-3A(vector)
- 104(C):RecA+1-3A(vector)
- 105(A):12(upstream)+101(downstream)+1-1G(vector)
- 106(A):12(upstream)+102(downstream)+1-1G(vector)
- Transformation of ligation products
September 12 (Mon)
- To liquid culture:103,104,105,106
September 13 (Tue)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 107(A):12(upstream)+103(downstream)+1-1G(vector)
- 108(A):12(upstream)+104(downstream)+1-1G(vector)
- Transformation of ligation products
September 14 (Wed)
- To liquid culture:107,108
September 15 (Thu)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:108
September 16 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
September 17 (Sat)
- Ligation
- 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
- 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
- 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
- 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
- Transformation of ligation products