Team:UQ-Australia/Notebook/August

From 2011.igem.org

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Revision as of 10:52, 4 October 2011

After still failing to produce any positive results from our cloning, we discovered that our parts had been incorrectly synthesized at this point. We sourced some genes from elsewhere and instead began focusing on other components of our system. We spent a lot of time this month finalizing other Outreach opportunities and doing work related to our Human Practices section, as well as developing the laboratory work.

Summary of Lab Work

We repeated the digestion, ligation and transformations of a number of out parts before determining the reason behind why these weren't working.

At this point, we turned to using PCR to amplify some parts from plasmids we had been provided by Dr Alex Ninfa and Dr Susan Rowland, with gels showing bands of the correct sizes.

We purified these pieces and digested them, but subsequent ligations into the plasmid backbone pSB1C3 failed.

30 Aug 2011 - gel electrophoresis and purification of the above digestions - did not obtain right sized bands for GlnAp2, Plac/ara or glnG. pSb1C3, LacI and GFP were purified.

31 Aug 2011 - re-transformation of LacI, GlnAp2 and Plac/ara from MrGene

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 {|style="width:100%;" border="0" cellpadding="10" cellspacing="0"
-|rowspan="2"|[HERE] Please type a summary of August here! [HERE]
+|rowspan="2"|After still failing to produce any positive results from our cloning, we discovered that our parts had been incorrectly synthesized at this point. We sourced some genes from elsewhere and instead began focusing on other components of our system. We spent a lot of time this month finalizing other Outreach opportunities and doing work related to our Human Practices section, as well as developing the laboratory work.
 ! width="125" |[[File:IGEM basic Logo stylized.png|125x125px|link=https://2011.igem.org]]
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-== '''<span style="color:#558822"> [HERE] Heading 1 [HERE] </span>''' ==
+== '''<span style="color:#558822"> Summary of Lab Work </span>''' ==
-[HERE] Start typing stuff here!! [HERE]
+We repeated the digestion, ligation and transformations of a number of out parts before determining the reason behind why these weren't working.
-== '''<span style="color:#558822"> [HERE] Heading 2 [HERE] </span>''' ==
+At this point, we turned to using PCR to amplify some parts from plasmids we had been provided by Dr Alex Ninfa and Dr Susan Rowland, with gels showing bands of the correct sizes.
-etc
+We purified these pieces and digested them, but subsequent ligations into the plasmid backbone pSB1C3 failed.
-etc
+Aug 2011 - gel electrophoresis and purification of the above digestions - did not obtain right sized bands for GlnAp2, Plac/ara or glnG. pSb1C3, LacI and GFP were purified.
+Aug 2011 - re-transformation of LacI, GlnAp2 and Plac/ara from MrGene