"

From 2011.igem.org

Revision as of 02:18, 29 September 2011 (view source) NatyNatz (Talk | contribs) ← Older edit		Latest revision as of 04:18, 29 September 2011 (view source) YaNa15 (Talk | contribs)
(3 intermediate revisions not shown)
Line 1:		Line 1:
-	We take a sample of rh1AB purifying gen without mutation and doing a PCR because doesn’t have a lot and we have to digest with Pst1. On Monday we are going to do a mutagenesis.	+	==22 Septembre 2011==
-	We use the PCR protocol that functions well with our gene ([https://2011.igem.org/PCR'''PCR Protocol''']) and we test all primers because we don’t know which primers the team used the last year for genes amplification.	+	'''We did:'''
		+
		+	1. Broth of XL10 gold (50 microliters in 2 ml of LB).
		+
		+	2. Was supplemented the NZCYM of sigma adding compounds to maik it NZY+.

---

Latest revision as of 04:18, 29 September 2011

22 Septembre 2011

We did:

1. Broth of XL10 gold (50 microliters in 2 ml of LB).

2. Was supplemented the NZCYM of sigma adding compounds to maik it NZY+.