From 2011.igem.org

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 {{Template:Team:Washington/Templates/Top}}
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+__NOTOC__
-INSERT INFO HERE... LOOK AT OTHER PAGES FOR EXAMPLES OF FORMATING AND INSERTING PICTURES.   CLICK THE EDIT BUTTON ON THE UPPER LEFT SIDE OF THE PAGE AFTER YOU HAVE SIGNED IN.
+<center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br>
-[https://2011.igem.org/Team:Washington/Alkanes/Protocols/Example_1 Example 1]
+=General Protocols=
-[https://2011.igem.org/Team:Washington/Alkanes/Protocols/Example_2 Example 2]
+[https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
+[https://2011.igem.org/Team:Washington/Protocols/PCR  General PCR Protocol]
-[https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagensis]
+[https://2011.igem.org/Team:Washington/Protocols/Digestion  General Digestion Protocol]
-[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
+[https://2011.igem.org/Team:Washington/Protocols/Ligation  General Ligation Protocol]
-[https://2011.igem.org/Team:Washington/Protocols/plate_expression  96 Well Plate Protein Expression]
+[https://2011.igem.org/Team:Washington/Protocols/Transformation  General Transformation Protocol]
-[http://soslab.ee.washington.edu/igem/2011/index.php/Projects:Biofuels/AlkaneBiosynthesis Alkane Biosynthesis Protocol]
+[https://2011.igem.org/Team:Washington/Protocols/Colony Colony PCR Protocol]
-[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
+[https://2011.igem.org/Team:Washington/Protocols/Competent  Competent Cell Prep Protocol]
-[https://2011.igem.org/Team:Washington/Protocols/gene_assembly Gene Assembly With Oligos]
+[https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis]
+[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
+[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
-=Protocol Page=
+[https://2011.igem.org/Team:Washington/Protocols/gene_assembly Gene Assembly With Oligos]
-'''restriction digest'''
-uL DNA
-uL buffer ( 2 for most, check  http://www.neb.com/nebecomm/DoubleDigestCalculator.asp)
-.5 uL BSA
-uL enzyme 1
-uL enzyme 2
-water to 50 uL(32.5 uL, add first)
-'''oligo assembly by PCR'''
-resuspend oligos with water, amount of water= concentration(in nm)*10 in uL
-make oligo mix with 5uL of each primer
-PCR reaction:
-uL phusion
-.5uL oligo mix
-uL first oligo
+[https://2011.igem.org/Team:Washington/Protocols/sequencing Sequencing]
-uL last oligo
+[https://2011.igem.org/Team:Washington/Protocols/CompDesign  Computational Protein Design]
-uL buffer
+[https://2011.igem.org/Team:Washington/Protocols/Glycerol_Stocks  Glycerol Stocks]
-uL dnTP
-dH20 to 50uL
+=Make It:  Diesel Production Protocols=
-'''Ligation'''
+[https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction]
-uL insert
+[https://2011.igem.org/Team:Washington/alkanebiosynthesis_cloning Alkane Biosynthesis cloning]
-uL vector
+[https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team]
-uL T4 ligase buffer
-uL T4 ligase
+=Break It:  Gluten Destruction Protocols=
-incubate at <s>37C</s> no.  **Note that NEB website recommends 16C - room temp for ligations, we do ours at RT and it works well.  1 hour.
+[https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Whole Cell Lysate Assay]
-'''Colony PCR with Green tag'''
+[https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification]
-Master mix(7ul):
+[https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay]
-ul 10uM forword primer
-ul 10uM reverse Primer
+=Make It: iGEM Toolkits=
-ul 2x Green tag
+[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]
-Cell water(3ul): Pick one colony from the plate and mix with 10ul of ddH2O to make 10ul of cell water
+[https://2011.igem.org/Team:Washington/Protocols/Elect. Electroporation (Transformation)]
-Reaction = Master mix(7ul) + Cell water(3ul) = 10ul per tube
+[https://2011.igem.org/Team:Washington/Protocols/Gib_Rxn Gibson Cloning/Assembly]
-Use program "Colony" & change the extention time (1min per kb)
+[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification]
-'''Heat Shock Transformation'''
+[https://2011.igem.org/Team:Washington/Protocols/High_PCR High-Yield PCR (Full-Gene Assembly)]
-ul ligation
+[https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)]
-ul cells
+[https://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)]
-Ice 20 minutes
+[https://2011.igem.org/Team:Washington/Protocols/pGA. pGA Vector Assay]
-Heat shock at 42C for 1 minute
+[https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol]
-Ice 2 minutes
+[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]
-Prepare 200 ul of TB (no anti) and transformed cells in culture tube
-Incubate at 37C for 1 hour
-Plate cells
+=Wiki Design=
+[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)]