Team:Washington/Protocols
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- | {{Template:Team:Washington/Templates/ | + | {{Template:Team:Washington/Templates/Top}} |
+ | __NOTOC__ | ||
- | + | <center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br> | |
+ | |||
+ | =General Protocols= | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis General Agarose Gel Electrophoresis] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/PCR General PCR Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Digestion General Digestion Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Ligation General Ligation Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Transformation General Transformation Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Colony Colony PCR Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Competent Competent Cell Prep Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis] | ||
+ | |||
+ | [http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/gene_assembly Gene Assembly With Oligos] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/sequencing Sequencing] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/CompDesign Computational Protein Design] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Glycerol_Stocks Glycerol Stocks] | ||
+ | |||
+ | |||
+ | =Make It: Diesel Production Protocols= | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/alkanebiosynthesis_cloning Alkane Biosynthesis cloning] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team] | ||
+ | |||
+ | |||
+ | =Break It: Gluten Destruction Protocols= | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Whole Cell Lysate Assay] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay] | ||
+ | |||
+ | |||
+ | =Make It: iGEM Toolkits= | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Elect. Electroporation (Transformation)] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Gib_Rxn Gibson Cloning/Assembly] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/High_PCR High-Yield PCR (Full-Gene Assembly)] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/pGA. pGA Vector Assay] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | ||
+ | |||
+ | |||
+ | |||
+ | =Wiki Design= | ||
+ | [https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] |
Latest revision as of 02:10, 29 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Cell Lysate Assay by Decarbonylase Redesign Team
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: iGEM Toolkits
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Induction Studies of Proteins Fusions (mam-sfGFP)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)