Team:WashU/Notebook

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{{WashUHeader2011}}
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:WashU_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:WashU_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:WashU | Team Example]]
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:WashU|Home]]
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!align="center"|[[Team:WashU/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=WashU Official Team Profile]
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!align="center"|[[Team:WashU/Project|Project]]
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!align="center"|[[Team:WashU/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:WashU/Modeling|Modeling]]
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!align="center"|[[Team:WashU/Notebook|Notebook]]
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!align="center"|[[Team:WashU/Safety|Safety]]
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!align="center"|[[Team:WashU/Attributions|Attributions]]
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==Notebook==
==Notebook==
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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[[File:Lab Notebook.jpg|300px|thumb|right|]]
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===May 31===
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#PCR shopping list
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[[Team:WashU/Notebook/May2011|Lab Notebook-May]]
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##PCR buffer w/o MgCl  - 5ML - $45.00
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[[Team:WashU/Notebook/June2011|Lab Notebook-June]]
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##M8787- 5ML -MgCl Reagent- $49.30
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##D7295-.5ML - dNTP - $73.00
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##D4184-250UN - Taq Polymerase - $150.00
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##D8045-250UN - AccuTaq - $300
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##E1385-5ML – Ethidium Bromide – $23.70
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##T8280-1L – Tris Acetate EDTA Buffer – $45.10
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##NA1020-1KT – PCR Clean-Up Kit -$102.50
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##NA1111-1KT -  Gel Extraction Kit - $102.00
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Subtotal: $890.60
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[[Team:WashU/Notebook/July2011|Lab Notebook-July]]
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*Meeting with Cohen grad students to finalize plan and get cassettes
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==June 1==
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[[Team:WashU/Notebook/August2011|Lab Notebook-August]]
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*Lesson from Burt on how to design primers
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[[Team:WashU/Notebook/September2011|Lab Notebook-September]]
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*Codon Optimized the four genes using tool at: http://www.encorbio.com/protocols/Codon.htm
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**Recheck gene and restriction sites to make sure enzyme does not cut in the middle of the gene
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*Design Primers
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*Safety Training
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Latest revision as of 04:02, 28 September 2011





Notebook

Lab Notebook.jpg

Lab Notebook-May

Lab Notebook-June

Lab Notebook-July

Lab Notebook-August

Lab Notebook-September