Team:WashU/Notebook/May2011

From 2011.igem.org





May 31

  • Preliminary Shopping List
    1. PCR buffer w/o MgCl - 5ML - $45.00
    2. M8787- 5ML -MgCl Reagent- $49.30
    3. D7295-.5ML - dNTP - $73.00
    4. D4184-250UN - Taq Polymerase - $150.00
    5. D8045-250UN - AccuTaq - $300
    6. E1385-5ML – Ethidium Bromide – $23.70
    7. T8280-1L – Tris Acetate EDTA Buffer – $45.10
    8. NA1020-1KT – PCR Clean-Up Kit -$102.50
    9. NA1111-1KT - Gel Extraction Kit - $102.00

Subtotal: $890.60

  • Met with Cohen Lab grad students to finalize plan and get cassettes.
  • Received Leu2 cassette in pRS305 plasmid.
  • Received Ura3 cassette in pRS306 plasmid.