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From 2011.igem.org

September 24

pSB1C3 plasmid arrived; genes ligated into plasmid by MolBio group Transformed E. coli LB plates: 150 mL h20 + 6.25 g LB broth + 3.75 g Bacto Agar to Erlenmeyer flask add water to 250 mL (final volume ended up being ~130 mL)

Chloramphenicol stock solution: 0.07 g chloramphenicol into 2 mL, filter-sterilize; add ~130 uL stock solution into LB (1:1000 dilution)