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Team:Washington/Alkanes/Parts
From 2011.igem.org
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The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry]. | The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry]. | ||
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=='''Composite'''== | =='''Composite'''== | ||
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The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production. | The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production. | ||
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=='''Coding'''== | =='''Coding'''== | ||
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 BBa_K590031 '''Synechococcus elongatus PCC7942 Aldehyde Decarbonylase'''] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 BBa_K590031 '''Synechococcus elongatus PCC7942 Aldehyde Decarbonylase'''] | ||
| - | This part encodes an enzyme | + | This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. This part has been codon-optimized for ''E. coli''. |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 BBa_K590032 '''Acyl-ACP Reductase'''] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 BBa_K590032 '''Acyl-ACP Reductase'''] | ||
This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for ''E. coli''. | This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for ''E. coli''. | ||
Latest revision as of 03:45, 28 September 2011
The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry].
Composite
[http://partsregistry.org/Part:BBa_K590025 BBa_K590025 "The Petrobrick"]
A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 AAR] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 ADC], each behind a standard Elowitz RBS. All of this in psb1C3 regulated by a high constitutive promoter.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590026 BBa_K590026 ADC-PSB1C3-High constitutive]
The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590027 BBa_K590027 AAR-PSB1C3-High consititutive]
The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
Coding
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 BBa_K590031 Synechococcus elongatus PCC7942 Aldehyde Decarbonylase]
This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. This part has been codon-optimized for E. coli.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 BBa_K590032 Acyl-ACP Reductase]
This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for E. coli.
