Team:Colombia/Notebook/Protocols

From 2011.igem.org

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===PCR CBP===
{| border="1" align="left" style="text-align:center;"
{| border="1" align="left" style="text-align:center;"
|Reactives
|Reactives
|1X µl
|1X µl
|3X µl
|3X µl
 +
|-
|H2O
|H2O
|18.8
|18.8
|56.4
|56.4
 +
|-
|Buffer PFX
|Buffer PFX
|2.5
|2.5
|7.5
|7.5
 +
|-
|MgSO4
|MgSO4
|0.5
|0.5
|1.5
|1.5
 +
|-
|dNTP´s
|dNTP´s
|0.75
|0.75
|2.25
|2.25
 +
|-
|FW (Pr3)
|FW (Pr3)
|0.75
|0.75
|2.25
|2.25
 +
|-
|RV (Pr4)
|RV (Pr4)
|0.75
|0.75
|2.25
|2.25
 +
|-
|PFX
|PFX
|0.2
|0.2
|0.6
|0.6
 +
|-
|ADN
|ADN
|0.75
|0.75
|2.25
|2.25
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|-
|Mix Volume
|Mix Volume
|25
|25
|75
|75
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|}
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===PCR Sensor===
 +
{| border="1" align="left" style="text-align:center;"
 +
|Reactives
 +
| µl
 +
|-
 +
|H2O
 +
|17.3
 +
|-
 +
|Buffer PFX
 +
|3
 +
|-
 +
|MgSO4
 +
|0.6
 +
|-
 +
|dNTP´s
 +
|0.9
 +
|-
 +
|FW (1)
 +
|0.9
 +
|-
 +
|RV (2)
 +
|0.9
 +
|-
 +
|PFX
 +
|0.4
 +
|-
 +
|Enhancer
 +
|3
 +
|
 +
|-
 +
|ADN
 +
|3
 +
|-
 +
|Total Mix
 +
|30
|}
|}

Latest revision as of 16:48, 27 September 2011

Template:Https://2011.igem.org/User:Tabima

Contents

Protocols

PCR for 16s (10 µl)

Reactives µl µl
Buffer 2.5 1
MgCl2 2 0.8
DNTP´s 0.5 0.2
P1492R 0.5 0.2
P27F 0.5 0.2
Taq 0.25 0.1
DNA 2 0.8
H2O 17.75 6.8
Final Vol 26 10















PCR Pfx

Reactives ul
H2O 17.2
Buffer PFX 3
MgSO4 0.6
dNTP´s 0.9
FW 0.9
RV 0.9
Enhancer 3
Taq PFX 0.5
ADN 3
Total Mix 30

















PCR CBP

Reactives 1X µl 3X µl
H2O 18.8 56.4
Buffer PFX 2.5 7.5
MgSO4 0.5 1.5
dNTP´s 0.75 2.25
FW (Pr3) 0.75 2.25
RV (Pr4) 0.75 2.25
PFX 0.2 0.6
ADN 0.75 2.25
Mix Volume 25 75
















PCR Sensor

Reactives µl
H2O 17.3
Buffer PFX 3
MgSO4 0.6
dNTP´s 0.9
FW (1) 0.9
RV (2) 0.9
PFX 0.4
Enhancer 3
ADN 3
Total Mix 30
Retrieved from "http://2011.igem.org/Team:Colombia/Notebook/Protocols"