Team:Colombia/Notebook/Protocols

From 2011.igem.org

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{{ https://2011.igem.org/User:Tabima }}
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----
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=Protocols=
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===PCR for 16s (10 µl)===
===PCR for 16s (10 µl)===
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{| border="1" align="center" style="text-align:center;"
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{| border="1" align="left" style="text-align:center;"
|Reactives
|Reactives
|µl
|µl
|µl
|µl
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|-
|Buffer  
|Buffer  
|2.5
|2.5
|1
|1
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|-
|MgCl2
|MgCl2
|2
|2
|0.8
|0.8
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|-
|DNTP´s
|DNTP´s
|0.5
|0.5
|0.2
|0.2
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|-
|P1492R
|P1492R
|0.5
|0.5
|0.2
|0.2
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|-
|P27F
|P27F
|0.5
|0.5
|0.2
|0.2
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|-
|Taq
|Taq
|0.25
|0.25
|0.1
|0.1
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|-
|DNA
|DNA
|2
|2
|0.8
|0.8
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|-
|H2O
|H2O
|17.75
|17.75
|6.8
|6.8
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|Total Mix
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|-
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|Final Vol
|26
|26
|10
|10
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|}
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===PCR Pfx===
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{| border="1" align="left" style="text-align:center;"
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|Reactives
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|ul
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|-
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|H2O
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|17.2
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|-
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|Buffer PFX
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|3
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|-
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|MgSO4
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|0.6
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|-
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|dNTP´s
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|0.9
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|-
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|FW
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|0.9
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|-
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|RV
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|0.9
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|-
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|Enhancer
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|3
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|-
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|Taq PFX
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|0.5
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|-
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|ADN
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|3
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|-
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|Total Mix
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|30
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|}
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===PCR CBP===
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{| border="1" align="left" style="text-align:center;"
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|Reactives
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|1X µl
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|3X µl
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|-
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|H2O
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|18.8
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|56.4
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|-
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|Buffer PFX
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|2.5
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|7.5
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|-
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|MgSO4
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|0.5
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|1.5
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|-
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|dNTP´s
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|0.75
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|2.25
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|-
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|FW (Pr3)
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|0.75
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|2.25
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|-
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|RV (Pr4)
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|0.75
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|2.25
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|-
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|PFX
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|0.2
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|0.6
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|-
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|ADN
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|0.75
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|2.25
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|-
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|Mix Volume
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|25
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|75
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|}
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===PCR Sensor===
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{| border="1" align="left" style="text-align:center;"
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|Reactives
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| µl
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|-
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|H2O
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|17.3
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|-
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|Buffer PFX
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|3
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|-
 +
|MgSO4
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|0.6
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|-
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|dNTP´s
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|0.9
 +
|-
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|FW (1)
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|0.9
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|-
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|RV (2)
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|0.9
 +
|-
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|PFX
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|0.4
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|-
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|Enhancer
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|3
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|
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|-
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|ADN
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|3
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|-
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|Total Mix
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|30
|}
|}

Latest revision as of 16:48, 27 September 2011

Template:Https://2011.igem.org/User:Tabima

Contents

Protocols

PCR for 16s (10 µl)

Reactives µl µl
Buffer 2.5 1
MgCl2 2 0.8
DNTP´s 0.5 0.2
P1492R 0.5 0.2
P27F 0.5 0.2
Taq 0.25 0.1
DNA 2 0.8
H2O 17.75 6.8
Final Vol 26 10















PCR Pfx

Reactives ul
H2O 17.2
Buffer PFX 3
MgSO4 0.6
dNTP´s 0.9
FW 0.9
RV 0.9
Enhancer 3
Taq PFX 0.5
ADN 3
Total Mix 30

















PCR CBP

Reactives 1X µl 3X µl
H2O 18.8 56.4
Buffer PFX 2.5 7.5
MgSO4 0.5 1.5
dNTP´s 0.75 2.25
FW (Pr3) 0.75 2.25
RV (Pr4) 0.75 2.25
PFX 0.2 0.6
ADN 0.75 2.25
Mix Volume 25 75
















PCR Sensor

Reactives µl
H2O 17.3
Buffer PFX 3
MgSO4 0.6
dNTP´s 0.9
FW (1) 0.9
RV (2) 0.9
PFX 0.4
Enhancer 3
ADN 3
Total Mix 30
Retrieved from "http://2011.igem.org/Team:Colombia/Notebook/Protocols"