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Team:Colombia/Notebook/Protocols
From 2011.igem.org
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===PCR CBP=== | ===PCR CBP=== | ||
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Revision as of 16:36, 27 September 2011
Template:Https://2011.igem.org/User:Tabima
Contents |
Protocols
PCR for 16s (10 µl)
| Reactives | µl | µl |
| Buffer | 2.5 | 1 |
| MgCl2 | 2 | 0.8 |
| DNTP´s | 0.5 | 0.2 |
| P1492R | 0.5 | 0.2 |
| P27F | 0.5 | 0.2 |
| Taq | 0.25 | 0.1 |
| DNA | 2 | 0.8 |
| H2O | 17.75 | 6.8 |
| Final Vol | 26 | 10 |
PCR Pfx
| Reactives | ul |
| H2O | 17.2 |
| Buffer PFX | 3 |
| MgSO4 | 0.6 |
| dNTP´s | 0.9 |
| FW | 0.9 |
| RV | 0.9 |
| Enhancer | 3 |
| Taq PFX | 0.5 |
| ADN | 3 |
| Total Mix | 30 |
PCR CBP
| Reactives | 1X µl | 3X µl |
| H2O | 18.8 | 56.4 |
| Buffer PFX | 2.5 | 7.5 |
| MgSO4 | 0.5 | 1.5 |
| dNTP´s | 0.75 | 2.25 |
| FW (Pr3) | 0.75 | 2.25 |
| RV (Pr4) | 0.75 | 2.25 |
| PFX | 0.2 | 0.6 |
| ADN | 0.75 | 2.25 |
| Mix Volume | 25 | 75 |
