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Team:Colombia/Notebook/Protocols
From 2011.igem.org
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===PCR for 16s (10 µl)=== | ===PCR for 16s (10 µl)=== | ||
| - | {| border="1" align=" | + | {| border="1" align="left" style="text-align:center;" |
|Reactives | |Reactives | ||
|µl | |µl | ||
| - | | µl | + | |µl |
|- | |- | ||
|Buffer | |Buffer | ||
Revision as of 16:13, 27 September 2011
PCR for 16s (10 µl)
| Reactives | µl | µl |
| Buffer | 2.5 | 1 |
| MgCl2 | 2 | 0.8 |
| DNTP´s | 0.5 | 0.2 |
| P1492R | 0.5 | 0.2 |
| P27F | 0.5 | 0.2 |
| Taq | 0.25 | 0.1 |
| DNA | 2 | 0.8 |
| H2O | 17.75 | 6.8 |
| Final Vol | 26 | 10 |
