Team:Colombia/Notebook/Protocols

From 2011.igem.org

(Difference between revisions)
(Created page with "===PCR for 16s (10 µl)=== {| border="1" align="center" style="text-align:center;" |Reactives |µl |µl |Buffer |2.5 |1 |MgCl2 |2 |0.8 |DNTP´s |0.5 |0.2 |P1492R |0.5 |0.2 |P27F...")
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|Reactives
|Reactives
|µl
|µl
-
|µl
+
| µl
 +
|-
|Buffer  
|Buffer  
|2.5
|2.5
|1
|1
 +
|-
|MgCl2
|MgCl2
|2
|2
|0.8
|0.8
 +
|-
|DNTP´s
|DNTP´s
|0.5
|0.5
|0.2
|0.2
 +
|-
|P1492R
|P1492R
|0.5
|0.5
|0.2
|0.2
 +
|-
|P27F
|P27F
|0.5
|0.5
|0.2
|0.2
 +
|-
|Taq
|Taq
|0.25
|0.25
|0.1
|0.1
 +
|-
|DNA
|DNA
|2
|2
|0.8
|0.8
 +
|-
|H2O
|H2O
|17.75
|17.75
|6.8
|6.8
-
|Total Mix
+
|-
 +
|Final Vol
|26
|26
|10
|10
|}
|}

Revision as of 16:12, 27 September 2011

PCR for 16s (10 µl)

Reactives µl µl
Buffer 2.5 1
MgCl2 2 0.8
DNTP´s 0.5 0.2
P1492R 0.5 0.2
P27F 0.5 0.2
Taq 0.25 0.1
DNA 2 0.8
H2O 17.75 6.8
Final Vol 26 10
Retrieved from "http://2011.igem.org/Team:Colombia/Notebook/Protocols"