Feasibility Analysis

We verified that our initial idea works and that we can actually get a moving GFP band in the channel responding to different input acetaldehyde concentrations!

For a 5cm long channel, in order for our SmoColi bacteria to start reporting the presence of acetaldehyde in the air (when the GFP band appears at the beginning of the channel), the system should be supplied with around 44.05 mg/L of acetaldehyde. In order for the band to be able to form at the very end of the channel, the input acetaldehyde concentration should be 2420 mg/L.

Figure 1: GFP (μM) vs. increasing acetaldehyde input (mg/L). The band appears when the acetaldehyde concentration is in the range of approximately 20-70 mg/L

Video 1: Steady state simulation sweep from 1 to 2500 mg/l acetaldehyde concentration in reservoir: 3D GFP concentration in mol/m3, 5 slices through the channel. Channel width: 2 mm, Channel length: 5 cm. Press play to start video!

Process Design

After simulating our system in COMSOL, we came to the following conclusions that helped us designing the channel experimentally:

• E. coli can generate a gradient by degrading a substance replenished by diffusion in an agarose-filled channel.
• No small microfluidics devise needed, no flow needed. The setup can be done in a small tube with diffusion only.
• It can do so within a reasonable experimental timeframe (within several hours).
• It can do so within reasonable channel lengths (several centimeters.
• Channel diameter is irrelevant for our system.
• As mentioned, for a 5cm long channel, we need 44.05 mg/L of input acetaldehyde concentration for the band to appear, 2420 mg/L for it to come to the end of the channel.