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Team:Freiburg/Notebook/18 June
From 2011.igem.org
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| - | == | + | {{:Team:Freiburg/Templates/header}} |
| - | - | + | <html> |
| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/17_June">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 18 June </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/19_June">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
| - | ==3A assembly: Ligation== | + | ==<span style="color:orange;">Lysis cassette</span>== |
| + | ===3A assembly: Ligation=== | ||
'''Investigators: Theo''' | '''Investigators: Theo''' | ||
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<span style="color:blue;">Comments: Ligation of digested parts from yesterday.</span> | <span style="color:blue;">Comments: Ligation of digested parts from yesterday.</span> | ||
| - | S11<br/> | + | S11: heat sensitive cI QPI with a high promoter preceding the repressor<br/> |
| - | S15<br/> | + | S15: Bacteriophage Holin Gene pS105<br/> |
| - | S1 | + | S1, S2, S3: promotor<br/> |
| + | S4, S5, S6: RBS<br/> | ||
Target vector | Target vector | ||
| - | ==Transformation== | + | ===Transformation=== |
'''Investigators: Theo''' | '''Investigators: Theo''' | ||
| - | Transformation of ligated parts. Incubation at 30°C. | + | Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor. |
| + | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 01:21, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
