Team:Freiburg/Notebook/17 June
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- | == | + | {{:Team:Freiburg/Templates/header}} |
+ | <html> | ||
+ | <div id="notebook-page-header"> | ||
+ | <div id="notebook-back" width="100px" > | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/16_June">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 17 June </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/18_June">Next entry</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> | ||
- | + | ==<span style="color:green;">green light receptor</span>== | |
+ | ===PCR with Ccar and Ccas=== | ||
- | == | + | '''Investigators: Julia''' |
+ | |||
+ | |||
+ | different primer used for PCR: | ||
+ | |||
+ | {| cellpadding="10" cellspacing="1" border="1" | ||
+ | | | ||
+ | |Primer | ||
+ | |- | ||
+ | |Ccas | ||
+ | |5 and 7 | ||
+ | |- | ||
+ | |Ccar | ||
+ | |4 and 6 | ||
+ | |} | ||
+ | <br/> | ||
+ | <br/> | ||
+ | |||
+ | ==<span style="color:orange;">Lysis cassette</span>== | ||
+ | ===3A assembly: Digestion=== | ||
+ | |||
+ | '''Investigators: Theo''' | ||
+ | |||
+ | Vector: Backbone chloramphenicol<br/> | ||
+ | partI: S11<br/> | ||
+ | partII: S15<br/> | ||
+ | |||
+ | {| cellpadding="10" cellspacing="0" border="1" | ||
+ | | | ||
+ | |Enzyme 1 | ||
+ | |Enzyme 2 | ||
+ | |- | ||
+ | |Vector | ||
+ | |EcoRI | ||
+ | |PstI | ||
+ | |- | ||
+ | |PartI | ||
+ | |EcoRI | ||
+ | |SpeI | ||
+ | |- | ||
+ | |PartII | ||
+ | |Xbal | ||
+ | |PstI | ||
+ | |} | ||
+ | <br/> | ||
+ | <br/> | ||
+ | |||
+ | ===Miniprep=== | ||
+ | |||
+ | '''Investigators: Theo''' | ||
+ | |||
+ | Minipreps of BBa_E0040, BBa_K124014, BBa_M30109 | ||
+ | {{:Team:Freiburg/Templates/footer}} |
Latest revision as of 01:21, 22 September 2011