Team:Freiburg/Notebook/10 August
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+ | <html> | ||
+ | <div id="notebook-page-header"> | ||
+ | <div id="notebook-back" width="100px" > | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/9_August">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 10 August </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/11_August">Next entry</a> | ||
+ | </div> | ||
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==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
- | === | + | ===Results from the Transformation=== |
- | '''Investigators: | + | '''Investigators: Jakob''' |
+ | Results are again negativ... | ||
+ | *To-do: Tomorrow we will have a call with the iGEM-Team from Uppsala Sweden, maybe they can give us some favour. | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
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|'''2 L23''' | |'''2 L23''' | ||
|} | |} | ||
+ | |||
+ | Named the samples, see above in table. | ||
+ | |||
+ | '''Transformation''' | ||
+ | |||
+ | Transformation of ligated part: | ||
+ | * 1pTd (PR1-pcyATd) | ||
+ | * 2pTd (PR2-pcyATd) | ||
+ | * 1pTb (PR1-pcyATb) | ||
+ | * 2pTb (PR2-pcyATb) | ||
+ | * 1L23 (PR1-Ho1T) | ||
+ | * 2L23 (PR2-Ho1T) | ||
+ | |||
+ | |||
+ | plated out on plates with kanamycin resistance. | ||
==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
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==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== | ||
- | === | + | ===Trafo=== |
+ | |||
+ | '''Investigators: Sophie''' | ||
+ | |||
- | + | Trafo of new 3A-assembly parts in amp vector. | |
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Latest revision as of 01:06, 22 September 2011