Team:Freiburg/Notebook/10 August

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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 10 August </a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===Results from the Transformation===
-
'''Investigators:NAME'''
+
'''Investigators: Jakob'''
 +
Results are again negativ...
 +
*To-do: Tomorrow we will have a call with the iGEM-Team from Uppsala Sweden, maybe they can give us some favour.
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
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'''Investigators: Sandra'''
'''Investigators: Sandra'''
-
Digstion of:
+
'''Digestion'''
-
*Pr1
+
*PR1 (pSB1C3)
-
*Pr2
+
*PR2 (pSB1C3)
-
*pcyA
+
*pcyA (pSB1T3)
-
#pcyATd
+
#pcyATd (pSB1T3)
-
#pcyATb
+
#pcyATb (pSB1T3)
-
*L23
+
*L23 (pSB1T3)
 +
*vector (pSB1K3)
 +
 
 +
 
 +
Amount of DNA and H20:
 +
 
 +
{| cellpadding="10" cellspacing="0" border="1"
 +
|Sample
 +
|500(ng)/DNA concentration (ng/μl)
 +
|H20 μl
 +
|-
 +
|pcyATd
 +
|2.8
 +
|35.2
 +
|-
 +
|pcyaTb
 +
|2.9
 +
|35.1
 +
|-
 +
|PR2
 +
|7.2
 +
|30.8
 +
|-
 +
|PR1
 +
|6.1
 +
|31.9
 +
|-
 +
|L23
 +
|4.8
 +
|33.2
 +
|-
 +
|pSB1K3
 +
|20
 +
|18
 +
|}
 +
 
 +
 
 +
Enzymes necessary for digestion:
 +
 
 +
{|cellpadding="10" cellspacing="0" border="1"
 +
|
 +
|PR1/PR2
 +
|pcyA/L23
 +
|vector
 +
|-
 +
|enzyme 1
 +
|EcoRI
 +
|XbaI
 +
|EcoRI
 +
|-
 +
|enzyme 2
 +
|SpeI
 +
|PstI
 +
|PstI
 +
|}
 +
 
 +
 
 +
-incubated for 1 hours at 37°C.<br/>
 +
-heated for 20 minutes at 80°C
 +
 
 +
 
 +
'''Ligation'''
 +
 
 +
Ligation of:
 +
 
 +
{|cellpadding="10" cellspacing="0" border="1"
 +
|
 +
|PcayTd
 +
|PcyATb
 +
|L23 (ho1)
 +
|-
 +
|PR1
 +
|'''1 pTd'''
 +
|'''1 pTb'''
 +
|'''1 L23'''
 +
|-
 +
|PR2
 +
|'''2 pTd'''
 +
|'''2 pTb'''
 +
|'''2 L23'''
 +
|}
 +
 
 +
Named the samples, see above in table.
 +
 
 +
'''Transformation'''
 +
 
 +
Transformation of ligated part:
 +
* 1pTd (PR1-pcyATd)
 +
* 2pTd (PR2-pcyATd)
 +
* 1pTb (PR1-pcyATb)
 +
* 2pTb (PR2-pcyATb)
 +
* 1L23 (PR1-Ho1T)
 +
* 2L23 (PR2-Ho1T)
 +
 
 +
 
 +
plated out on plates with kanamycin resistance.
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===Trafo===
 +
 
 +
'''Investigators: Sophie'''
 +
 
-
'''Investigators: NAME'''
+
Trafo of new 3A-assembly parts in amp vector.
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:06, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

green light receptor

Results from the Transformation

Investigators: Jakob

Results are again negativ...

  • To-do: Tomorrow we will have a call with the iGEM-Team from Uppsala Sweden, maybe they can give us some favour.

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

3A-assembly

Investigators: Sandra

Digestion

  • PR1 (pSB1C3)
  • PR2 (pSB1C3)
  • pcyA (pSB1T3)
  1. pcyATd (pSB1T3)
  2. pcyATb (pSB1T3)
  • L23 (pSB1T3)
  • vector (pSB1K3)


Amount of DNA and H20:

Sample 500(ng)/DNA concentration (ng/μl) H20 μl
pcyATd 2.8 35.2
pcyaTb 2.9 35.1
PR2 7.2 30.8
PR1 6.1 31.9
L23 4.8 33.2
pSB1K3 20 18


Enzymes necessary for digestion:

PR1/PR2 pcyA/L23 vector
enzyme 1 EcoRI XbaI EcoRI
enzyme 2 SpeI PstI PstI


-incubated for 1 hours at 37°C.
-heated for 20 minutes at 80°C


Ligation

Ligation of:

PcayTd PcyATb L23 (ho1)
PR1 1 pTd 1 pTb 1 L23
PR2 2 pTd 2 pTb 2 L23

Named the samples, see above in table.

Transformation

Transformation of ligated part:

  • 1pTd (PR1-pcyATd)
  • 2pTd (PR2-pcyATd)
  • 1pTb (PR1-pcyATb)
  • 2pTb (PR2-pcyATb)
  • 1L23 (PR1-Ho1T)
  • 2L23 (PR2-Ho1T)


plated out on plates with kanamycin resistance.

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Trafo

Investigators: Sophie


Trafo of new 3A-assembly parts in amp vector.

                       

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