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Team:Freiburg/Notebook/18 June
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/17_June">Previous entry</a> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 18 June </a> | ||
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| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/19_June">Next entry</a> | ||
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==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
===3A assembly: Ligation=== | ===3A assembly: Ligation=== | ||
Revision as of 00:46, 22 September 2011
Lysis cassette
3A assembly: Ligation
Investigators: Theo
Comments: Ligation of digested parts from yesterday.
S11: heat sensitive cI QPI with a high promoter preceding the repressor
S15: Bacteriophage Holin Gene pS105
S1, S2, S3: promotor
S4, S5, S6: RBS
Target vector
Transformation
Investigators: Theo
Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.
