Team:ETH Zurich/Achievements/Model Results

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= Feasibility Analysis =
= Feasibility Analysis =
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We verified that our initial idea works and that we can actually get a moving GFP band in the channel responding to different input acetaldehyde concentrations. In order our smoColi bacteria to start reporting acetaldehyde in the air (when GFP band appears at the begining of the channel), system should be supplied with around 44.05 mg/L acetaldehyde. In order for the band to be able to form at the very end of the channel, the input acetaldehyde concentration should be 2420 mg/L. Above this concentration the alarm is turned on and the channel goes red.
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We verified that our initial idea works and that we can actually get a moving GFP band in the channel responding to different input acetaldehyde concentrations. In order our smoColi bacteria to start reporting acetaldehyde in the air (when GFP band appears at the begining of the channel), system should be supplied with around 44.05 mg/L acetaldehyde. In order for the band to be able to form at the very end of the channel, the input acetaldehyde concentration should be 2420 mg/L.
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Revision as of 00:34, 22 September 2011

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Modeling Results
Feasibility Analysis Process Design
By modeling and simulating our system we verified that it can actually be implemented in reality. We checked its feasibility, drew some conclusions and decided on a most suitable channel design.

Feasibility Analysis

We verified that our initial idea works and that we can actually get a moving GFP band in the channel responding to different input acetaldehyde concentrations. In order our smoColi bacteria to start reporting acetaldehyde in the air (when GFP band appears at the begining of the channel), system should be supplied with around 44.05 mg/L acetaldehyde. In order for the band to be able to form at the very end of the channel, the input acetaldehyde concentration should be 2420 mg/L.

Process Design

After simulating our system in COMSOL, we came to the following conclusions that helped us designing the channel experimentally:

  • E. coli can generate a gradient by degrading a substance replenished by diffusion in an agarose-filled channel.
  • It can do so within a reasonable experimental timeframe (within several hours).
  • It can do so within reasonable channel lengths (several centimeters.
  • Channel diameter is irrelevant for our system.
  • We need 44.05 mg/L of input acetaldehyde concentration for the band to appear, 2420 mg/L for it to come to the end of the channel.
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