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Team:Lyon-INSA-ENS/Realisation/ProtocolsFr
From 2011.igem.org
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| + | <div style="float : left; margin-top : -10px; margin-left : -200px"> | ||
| + | <a href="https://2011.igem.org/Main_Page" > | ||
| + | <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" /> | ||
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| - | + | Les antibiotiques ont été utilisés aux concentrations suivantes : <br> | |
Ampiciline (Amp) : 100µg/mL <br> | Ampiciline (Amp) : 100µg/mL <br> | ||
Chloramphenicol (Cm) : 20µg/mL <br> | Chloramphenicol (Cm) : 20µg/mL <br> | ||
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<br/> <br/> | <br/> <br/> | ||
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<p> <font color="green" size="5"> | <p> <font color="green" size="5"> | ||
| - | Choisissez l'un de nos protocoles pour lire sa description | + | Choisissez l'un de nos protocoles pour lire sa description<br><HR> |
| - | + | </font></p> | |
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| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuantFr"> Quantification de biofilms </a> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuantFr"> Quantification de biofilms <img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
</p> | </p> | ||
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<p> . | <p> . | ||
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_transFr"> Transformation chimique au CaCl2 </a> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_transFr"> Transformation chimique au CaCl2 <img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
</p> | </p> | ||
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<p> . | <p> . | ||
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/FermentasFr"> Digestion Fermentas </a> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/FermentasFr">Digestion Fermentas <img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
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| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/LigationFr"> Ligation </a> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/LigationFr"> Ligation <img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
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| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/IsolationFr"> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/IsolationFr"> Kit de miniprepMacherey-Nagel <img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
</p> | </p> | ||
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| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/OzymeFr"> Digestion Ozyme</a> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/OzymeFr"> Digestion Ozyme<img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
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| - | <p> . | + | |
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purificationFr"> | + | <p> . |
| + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/PouringFr"> Coulage de boîtes<img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> | ||
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| + | <br/> <br/> | ||
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| + | <p> . | ||
| + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purificationFr"> Kit de midiprep QIAGen <img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> | ||
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| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/MiniprepFr"> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/MiniprepFr"> Kit de miniprep QIAGen<img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
</p> | </p> | ||
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<br/> <br/> | <br/> <br/> | ||
| + | <p> . | ||
| + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/StorageFr"> Mise en collection de souches<img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> | ||
| + | </p> | ||
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| + | <br/> <br/> | ||
| + | <br/> <br/> | ||
<p> . | <p> . | ||
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_transFr"> Transformation chimique au TSS </a> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_transFr"> Transformation chimique au TSS <img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a> |
</p> | </p> | ||
| - | + | <br/> <br/><br/> <br/> | |
</div> | </div> | ||
</body> | </body> | ||
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{{Lyon-INSA-ENS/footer}} | {{Lyon-INSA-ENS/footer}} | ||
Latest revision as of 23:24, 21 September 2011
Conditions générales de culture
Les antibiotiques ont été utilisés aux concentrations suivantes :
Ampiciline (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycine (Spc) : 100µg/mL
Kanamycine (Kan) : 30µg/mL
Pour le chloramphenicol, le solvant est un mélange 70/30 (v/v) eau/éthanol ; les autres antibiotiques ont été dissouts dans l'eau. Tous les volumes sont calculés pour une solution mère 10X.
La composition des différents milieux que nous avons utilisés est la suivante :
LB : 10 g de tryptone, 5 g d'extarit de levure et 10g de NaCl pour 1L de LB liquide. Le LB solide pour les boîtes est réalisé par addition d'agar pour atteindre une concentration de 1.5%.
M63G : 100/1/1 (v/v/v) de M63, glucose 20% et LB.
LB/2 : un mélange 50/50 (v/v) de LB et eau.
Choisissez l'un de nos protocoles pour lire sa description
.
Transformation chimique au CaCl2 
.
Ligation
.
Kit de miniprepMacherey-Nagel
.
Mise en collection de souches
.
Transformation chimique au TSS
