Team:UNIPV-Pavia/Parts/DataPage

From 2011.igem.org

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<nowiki><div class="art-postcontent"><h1><span class="mw-headline"> <b>Data For Our Favorite New Parts</b> </span></h1></div></nowiki>  
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<div><h1><span> <b>Data For Our Favorite New Parts</b> </span></h1></div>
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<div><h2><span> <b>BBa_K516210</b> </span></h2></div>
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<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K516210</b> </span></h2></div></nowiki>
 
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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516210">BBa_K516210</a> (p<sub>Tet</sub>-RBS30-LuxI) - HSL synthesis device, aTc inducible. <br> This is one of four systems with different RBSs (output modulation) tested to quantify the produced HSL. The results obtained by testing this part allowed the identification of important model parameters to predict the behavior of the whole CTRL+E circuit.
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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516210">BBa_K516210</a> <b>(p<sub>Tet</sub>-RBS30-LuxI) - HSL synthesis device, aTc inducible.</b> <br> This is one of four systems with different RBSs (output modulation) tested to quantify the produced HSL. The results obtained by testing this part allowed the identification of important model parameters to predict the behavior of the whole CTRL+E circuit.
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<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K516334</b> </span></h2></div></nowiki>  
<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K516334</b> </span></h2></div></nowiki>  
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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516334">BBa_K516334</a> (p<sub>Lambda</sub>-RBS30-LuxR-T-p<sub>Lux</sub>-RBS34-mRFP-TT) - 3OC<sub>6</sub>-HSL biosensor, mRFP output <br>
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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516334">BBa_K516334</a> <b>(p<sub>Lambda</sub>-RBS30-LuxR-T-p<sub>Lux</sub>-RBS34-mRFP-TT) - 3OC<sub>6</sub>-HSL biosensor, mRFP output. </b> <br>
This is one of the four biosensors (different RBSs) built and characterized. This measurement system senses the HSL concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.  
This is one of the four biosensors (different RBSs) built and characterized. This measurement system senses the HSL concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.  
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<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K516230</b> </span></h2></div></nowiki>  
<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K516230</b> </span></h2></div></nowiki>  
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<!--NB: pLux in figure is pTet!!-->
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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516230">BBa_K516230</a> (p<sub>Tet</sub>-RBS34-mRFP-TT) - aTc inducible, mRFP output <br>
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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516230">BBa_K516230</a> <b>(p<sub>Tet</sub>-RBS34-mRFP-TT) - aTc inducible, mRFP output. </b><br>
This is one of the four p<sub>Tet</sub> measurement systems (different RBSs) built and characterized. This measurement system senses the aTc concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.  
This is one of the four p<sub>Tet</sub> measurement systems (different RBSs) built and characterized. This measurement system senses the aTc concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.  
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RBSs were used for the fine tuning of CTRL+E. Different experimental conditions were assayed.
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<b>RBSs</b><i> (based on Ron Weiss thesis)</i> were used for the fine tuning of CTRL+E. Different experimental conditions were assayed.
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<li><a href='http://partsregistry.org/Part:BBa_K081022:Experience'>Experience page of BBa_K081022</a>
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<li><a href='http://partsregistry.org/Part:BBa_K081022:Experience'>Experience page of BBa_K081022</a> <b> Plambda regulated luxR generator and Plux.</b>
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This device has been characterized with several RBSs in order to modulate the output range. In this way, this device can be used to tightly control the expression of downstream encoded protein from a very weak production (using weak RBS) to a very intensive production (with strong RBSs).  <br><br>The Hill's estimated parameters are reported in the table below:
This device has been characterized with several RBSs in order to modulate the output range. In this way, this device can be used to tightly control the expression of downstream encoded protein from a very weak production (using weak RBS) to a very intensive production (with strong RBSs).  <br><br>The Hill's estimated parameters are reported in the table below:
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<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_R0040</b> </span></h2></div></nowiki>  
<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_R0040</b> </span></h2></div></nowiki>  
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<li><a href='http://partsregistry.org/Part:BBa_R0040:Experience'>Experience page of BBa_R0040</a> <b> TetR repressible promoter.</b>
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pTet promoter was characterized with various RBSs and mRFP.  
pTet promoter was characterized with various RBSs and mRFP.  
<br><br>The Hill's estimated parameters are reported in the table below:
<br><br>The Hill's estimated parameters are reported in the table below:
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Data are provided as average [CV%]
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<div align="center">Data are provided as average [CV%]</div>
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The operative parameters are reported in the table below:
The operative parameters are reported in the table below:
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<li><a href='http://partsregistry.org/Part:BBa_K516999'>BBa_K516999</a>
<li><a href='http://partsregistry.org/Part:BBa_K516999'>BBa_K516999</a>
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This vector was designed and realized in order to facilitate the cloning of coding sequences downstream of the strong promoter p<sub>Tet</sub>.
This vector was designed and realized in order to facilitate the cloning of coding sequences downstream of the strong promoter p<sub>Tet</sub>.
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<nowiki><div class="art-postcontent"><h1><span class="mw-headline"> <b>We've Also Characterized the Following Parts</b> </span></h1></div></nowiki>  
<nowiki><div class="art-postcontent"><h1><span class="mw-headline"> <b>We've Also Characterized the Following Parts</b> </span></h1></div></nowiki>  
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For a detailed description of all the parts characterized, please visit the <a href='https://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized'>Parts Characterized</a> wiki page.
For a detailed description of all the parts characterized, please visit the <a href='https://2011.igem.org/Team:UNIPV-Pavia/Parts/Characterized'>Parts Characterized</a> wiki page.

Latest revision as of 22:55, 21 September 2011

UNIPV TEAM 2011

Data Page

Data For Our Favorite New Parts

BBa_K516210




BBa_K516210 (pTet-RBS30-LuxI) - HSL synthesis device, aTc inducible.
This is one of four systems with different RBSs (output modulation) tested to quantify the produced HSL. The results obtained by testing this part allowed the identification of important model parameters to predict the behavior of the whole CTRL+E circuit.



BBa_K516334


BBa_K516334 (pLambda-RBS30-LuxR-T-pLux-RBS34-mRFP-TT) - 3OC6-HSL biosensor, mRFP output.
This is one of the four biosensors (different RBSs) built and characterized. This measurement system senses the HSL concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.



BBa_K516230




BBa_K516230 (pTet-RBS34-mRFP-TT) - aTc inducible, mRFP output.
This is one of the four pTet measurement systems (different RBSs) built and characterized. This measurement system senses the aTc concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.



Data For existing Parts

RBSs from the community collection


RBSs (based on Ron Weiss thesis) were used for the fine tuning of CTRL+E. Different experimental conditions were assayed.

Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter:

RBS effpLux effpTet effJ23101 Declared efficiency
B00300.401.68142.450,6
B00310.01ND0.040,07
B00320.190.41930.400,3
B00341111


Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA or LuxI:

RBS effmRFP effAiiA effLuxI Declared efficiency
B00301.720.530.450,6
B00310.030.830.0280,07
B00320.370.50N.D.0.3
B00341111



BBa_K081022


This device has been characterized with several RBSs in order to modulate the output range. In this way, this device can be used to tightly control the expression of downstream encoded protein from a very weak production (using weak RBS) to a very intensive production (with strong RBSs).

The Hill's estimated parameters are reported in the table below:

RBS αpLux [(AUr/min)/cell] δpLux [-] ηpLux [-] kpLux [ng/ml]
BBa_B0030 438 [10] 0.05 [>100] 2 [47] 1.88 [27]
BBa_B0031 9.8 [7] 0.11 [57] 1.2 [29] 1.5 [26]
BBa_B0032 206 [3] 0 [>>100] 1.36 [10] 1.87 [9]
BBa_B0034 1105 [6] 0.02 [>100] 1.33 [19] 2.34 [18]
Data are provided as average [CV%].



The operative parameters are reported in the table below:

RBS RPUmax RPUmin Switch point [nM] Linear boundaries [MIN; MAX] [nM]
B00304.280.201.08[0.36; 3.27]
B00314.930.550.25[0.03; 2.30]
B00329.490.020.47[0.07; 3.07]
B003421.530.510.53[0.08; 3.77]



BBa_R0040



pTet promoter was characterized with various RBSs and mRFP.

The Hill's estimated parameters are reported in the table below:

RBS αpTet [(AUr/min)/cell] δpTet [-] ηpTet [-] kpTet [nM]
BBa_B0030 230.67 [3.7] 0.028 [91.61] 4.61 [23.73] 8.75 [4.16]
BBa_B0031 ND ND ND ND
BBa_B0032 55.77 [12] 1.53E-11 [>>100] 4.98 [57.62] 7.26 [14.98]
BBa_B0034 120 [5.95] 0.085 [40.6] 24.85 [47.6] 9 [5.43]
Data are provided as average [CV%]


The operative parameters are reported in the table below:

RBS RPUmax RPUmin Switch point [ng/ml] Linear boundaries [MIN; MAX] [ng/ml]
B00301.53~07.95[4.66;11.99]
B0031NDNDNDND
B00323.16~06.7[4.45;10.05]
B00342.730.238.96[8.27;9.71]



Improved existing parts

BBa_K516999




This vector was designed and realized in order to facilitate the cloning of coding sequences downstream of the strong promoter pTet.



We've Also Characterized the Following Parts


For a detailed description of all the parts characterized, please visit the Parts Characterized wiki page.

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia/Parts/DataPage"