"
Team:Lyon-INSA-ENS/Realisation/Week7Fr
From 2011.igem.org
(Difference between revisions)
(Created page with "{{Lyon-INSA-ENS/header}} {{Lyon-INSA-ENS/blocStyle}} {{INSA-Lyon/styletestaurelie}} {{Lyon-INSA-ENS/menuhorizontalFr}} {{Lyon-INSA-ENS/menuNotebookW7Fr}} <html> <body> <div cl...") |
|||
| (12 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
{{Lyon-INSA-ENS/blocStyle}} | {{Lyon-INSA-ENS/blocStyle}} | ||
{{INSA-Lyon/styletestaurelie}} | {{INSA-Lyon/styletestaurelie}} | ||
| - | {{Lyon-INSA-ENS/ | + | {{Lyon-INSA-ENS/menuhorizontalFrNotebook}} |
| - | {{Lyon-INSA-ENS/menuNotebookW7Fr}} | + | <!--{{Lyon-INSA-ENS/menuNotebookW7Fr}}--> |
| + | {{Lyon-INSA-ENS/menuCarnetDeBordVertical|Week 7 = actif}} | ||
<html> | <html> | ||
<body> | <body> | ||
| + | |||
| + | <div style="float : left; margin-top : -10px; margin-left : -200px"> | ||
| + | <a href="https://2011.igem.org/Main_Page" > | ||
| + | <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" /> | ||
| + | </a> | ||
| + | </div> | ||
| + | |||
| + | <div id="language";> | ||
| + | <img src="https://static.igem.org/mediawiki/2011/e/ef/Drapeau-anglais.gif"; width=20px; /> <a href="/Team:Lyon-INSA-ENS/Realisation/Week7">English version </a> | ||
| + | </div> | ||
<div class="contenugrand2";> | <div class="contenugrand2";> | ||
| Line 13: | Line 24: | ||
<br/> <br/> | <br/> <br/> | ||
<br/> <br/> | <br/> <br/> | ||
| + | <br> | ||
<div class="cadre" ; style="background-color:green;" > | <div class="cadre" ; style="background-color:green;" > | ||
| Line 20: | Line 32: | ||
<br/> | <br/> | ||
| - | <p style="text-align : center"> <small> | + | <p style="text-align : center"> <small> Du Lundi 25 Juillet au Vendredi 29 Juillet 2011 </small> </p> |
<br/> <br/> | <br/> <br/> | ||
| Line 26: | Line 38: | ||
<br/> <br/> | <br/> <br/> | ||
| - | <h6 style="text-align :left"> | + | <h6 style="text-align :left"> Lundi</h6> <HR> |
<br/> | <br/> | ||
| Line 36: | Line 48: | ||
</p> | </p> | ||
| + | <br/> <br/> | ||
<br/> <br/> | <br/> <br/> | ||
| - | <h6 style="text-align :left"> | + | <h6 style="text-align :left"> Mardi </h6> <HR> |
| - | <br/> | + | <br/> |
<p style=" line-height : 1.5em"> | <p style=" line-height : 1.5em"> | ||
| Line 50: | Line 63: | ||
| - | <h6 style="text-align :left"> | + | <h6 style="text-align :left"> Mercredi </h6> <HR> |
<br/> | <br/> | ||
<p style=" line-height : 1.5em"> | <p style=" line-height : 1.5em"> | ||
| - | PCR | + | Les PCR n'ont pas marché. |
</p> | </p> | ||
| + | <br/> <br/> | ||
<br/> <br/> | <br/> <br/> | ||
| - | <h6 style="text-align :left"> | + | <h6 style="text-align :left"> Jeudi </h6><HR> |
| - | + | ||
<br/> | <br/> | ||
| - | |||
<p style=" line-height : 1.5em"> | <p style=" line-height : 1.5em"> | ||
| Line 72: | Line 84: | ||
<br/> <br/> | <br/> <br/> | ||
| - | <h6 style="text-align :left"> | + | <h6 style="text-align :left"> Vendredi </h6><HR> |
<p style=" line-height : 1.5em"> | <p style=" line-height : 1.5em"> | ||
| - | + | La PCR a finalement marché avec la Phusion.<br> | |
</p> | </p> | ||
Latest revision as of 20:05, 21 September 2011
Semaine 7
Du Lundi 25 Juillet au Vendredi 29 Juillet 2011
Lundi
Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.
Miniprep of bacteria with mutated part CsgAB. Digestion to check restriction profile (removal of intern PstI site).
Mardi
Send of plasmid with the right profile for part CsgAB to sequencing. Extraction of new MC4100 strain's DNA. PCR with fresh DNA and Taq Phusion.
Mercredi
Les PCR n'ont pas marché.
Jeudi
Vendredi
La PCR a finalement marché avec la Phusion.
