Team:Osaka/week5

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Contents 1 August 28(Sun) 2 August 29(Mon) 3 August 30(Tue) 4 August 31(Wed) 5 September 1(Thu) 6 September 2(Fri) 7 September 3(Sat)

August 28(Sun)

1. Transfer to liquid culture:09,10,11
2. spectrophotometry of 04

August 29(Mon)

iGEM Japan Meet-Up at Kyoto University

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Gel electrophoresis of digests
   • 09 ok
   • 10 ok
   • 11 ×
4. Transformaton of Registry parts (See Table 6).

Table6

ID	Part Name	Resistance	Description
3-10C	<bbpart>BBa_K115056</bbpart>	AC	IPP -> OPP or DMAPP -> OPP

August 30(Tue)

1. To liquid culture:11,3-10C

August 31(Wed)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Gel electrophoresis of digests
   • 11,3-10C ok
4. Ligation
   • 12(C):1-1D(upstream)+1-2M(downstream)+1-3A(vector)
   • 13(C):1-9N(upstream)+1-2M(downstream)+1-3A(vector)
   • 14(A):3-10C(upstream)+2-18H(downstream)+1-5A(vector)
   • 15(C):2-16N(upstream)+1-23L(downstream)+1-3A(vector)
   • 16(A):3-10C(upstream)+3-6H(downstream)+1-5A(vector)
5. Transformation of ligation products

September 1(Thu)

1. Transfer to liquid culture:12,13,14,15,16

September 2(Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Gel electrophoresis of digests
   • 12,13,15 ok
   • 14,16 ×
4. Ligation
   • 17(A):15(upstream)+06(downstream)+1-1G(vector)
5. Transfer to liquid culture:03,04,3-10C

September 3(Sat)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Gel electrophoresis of digests
   • 3-10C × deactivation of SpeⅠ?
4. Inoculation of 03,04

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