"
Team:ETH Zurich
From 2011.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
|} | |} | ||
| - | | | + | {|class="roundContainer" |
| - | + | ||
[[File:Overview.jpg|600px|center|thumb|Overview of our system]] | [[File:Overview.jpg|600px|center|thumb|Overview of our system]] | ||
| - | | | + | | |
{|class="roundContainer" | {|class="roundContainer" | ||
|Link to Data Page goes here | |Link to Data Page goes here | ||
| | | | ||
Revision as of 22:55, 19 September 2011
| SmoColi |
| SmoColi is a bacterio-quantifier of acetaldehyde concentration that can be used as a passive smoke detector. Acetaldehyde is a toxic and carcinogenic component of cigarette smoke. It has a boiling point of 20.2 °C and is very volatile, thus can be used as an information carrier through air. The SmoColi cells are immobilized in an agarose-filled microfluidic device. The test solution is fed to one end of a microfluidic channel, in which an acetaldehyde gradient is established by diffusion and synthetic cellular degradation. The cells are engineered to sense acetaldehyde by a synthetically re-designed fungal acetaldehyde-responsive transactivator. The sensor is linked to a band-pass filter that drives GFP expression. This allows establishment of an input-concentration-dependent, moving fluorescent band displaying quantitative information about acetaldehyde. Finally, if the acetaldehyde concentration exceeds the threshold of malignance, a quorum-sensing-based mCherry alarm system springs into action, turning the entire device red. |
|
