Team:UNIPV-Pavia

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<h2 class="art-postheader">The project</h2>
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<p align='justify'>Our work aims at implementing the engineering concept of closed-loop control in <em>E. coli</em>, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around  a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it.
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To observe the desired behaviour, a fine tuning of the system was necessary.  The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:UNIPV-Pavia_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:UNIPV-Pavia_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:UNIPV-Pavia | Team Example]]
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{{end}}
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!align="center"|[[Team:UNIPV-Pavia|Home]]
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!align="center"|[[Team:UNIPV-Pavia/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=UNIPV-Pavia Official Team Profile]
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!align="center"|[[Team:UNIPV-Pavia/Project|Project]]
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!align="center"|[[Team:UNIPV-Pavia/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UNIPV-Pavia/Modeling|Modeling]]
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!align="center"|[[Team:UNIPV-Pavia/Notebook|Notebook]]
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!align="center"|[[Team:UNIPV-Pavia/Safety|Safety]]
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!align="center"|[[Team:UNIPV-Pavia/Attributions|Attributions]]
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Latest revision as of 13:14, 19 September 2011

UNIPV TEAM 2011


The project

Our work aims at implementing the engineering concept of closed-loop control in E. coli, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.


You can add captions too…



Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia"