Team:UNIPV-Pavia

From 2011.igem.org

(Difference between revisions)
 
(61 intermediate revisions not shown)
Line 1: Line 1:
{{main}}
{{main}}
<html>
<html>
-
  <h2 class="art-postheader">
+
 
-
Ttile
+
<table align='center' width='100%'>
-
</h2>
+
<tr>
 +
<td>
 +
<div style='text-align:center'><div class="thumbinner" style="width:100%;">
 +
<img alt="" src="https://static.igem.org/mediawiki/2011/b/b1/UNIPV_Pavia_Work_in_progress_scribblings.jpg" class="thumbimage" width="93%"></a></div></div>
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h2 class="art-postheader">The project</h2>
<div class="cleared"></div>
<div class="cleared"></div>
<div class="art-postcontent">
<div class="art-postcontent">
-
<p style="text-align:left;">
+
<p align='justify'>Our work aims at implementing the engineering concept of closed-loop control in <em>E. coli</em>, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around  a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it.
 +
To observe the desired behaviour, a fine tuning of the system was necessary.  The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.
 +
</p>
 +
<br>
 +
 
 +
 
 +
 
 +
 
 +
<link rel="stylesheet" href="https://2011.igem.org/Team:UNIPV-Pavia/Templates/nivo_default_css?action=raw&ctype=text/css" type="text/css"/>
 +
<link rel="stylesheet" href="https://2011.igem.org/Team:UNIPV-Pavia/Templates/nivo_css?action=raw&ctype=text/css" type="text/css"/>
 +
<script type="text/javascript" src="https://2011.igem.org/Team:UNIPV-Pavia/Templates/jquery_js?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2011.igem.org/Team:UNIPV-Pavia/Templates/nivo_slider_pack?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript">
 +
    $(window).load(function() {
 +
        $('#slider').nivoSlider({
 +
effect:'fade',
 +
pauseOnHover:true
 +
});
 +
    });
 +
</script>
 +
 
 +
 
 +
 
 +
 
 +
<div style="width:550px;margin:10px auto;">
 +
<div class="slider-wrapper theme-default">
 +
<div class="ribbon"></div>
 +
 
 +
<div id="nivoslider-125" class="nivoSlider" style="width:550px;height:366px;">
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/2/2d/UNIPV_Hometeam1.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/0/0a/UNIPV_Hometeam2.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/e/e1/UNIPV_Hometeam3.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f2/UNIPV_Hometeam4.jpg" title="Work in progress" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/9/9b/UNIPV_Home1.jpg" title="Pavia and the river Ticino" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/a/a2/UNIPV_Home2.jpg" title="University of Pavia" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f7/UNIPV_Home3.jpg" title="Faculty of Engineering" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f0/UNIPV_Home4.jpg" title="Scientific campus" alt="" />
 +
 
 +
</div>
 +
</div>
 +
<div id="nivoslider-125-caption-0" class="nivo-html-caption">You can add captions too&#8230;</div>
 +
<p><script type="text/javascript">
 +
jQuery(window).load(function(){
 +
    jQuery("#nivoslider-125").nivoSlider({
 +
        effect:"random",
 +
        slices:15,
 +
        boxCols:8,
 +
        boxRows:4,
 +
        animSpeed:500,
 +
        pauseTime:3000,
 +
        startSlide:0,
 +
        directionNav:true,
 +
        directionNavHide:true,
 +
        controlNav:true,
 +
        controlNavThumbs:false,
 +
        controlNavThumbsFromRel:true,
 +
        keyboardNav:true,
 +
        pauseOnHover:true,
 +
        manualAdvance:false
 +
    });
 +
});
 +
</script>
 +
</div>
 +
 
 +
<br><br>
-
<img width="370" height="264" alt="" src="https://static.igem.org/mediawiki/2011/1/19/Unipv11_mainimage.jpg" style="float:left;" /></p>
 
-
<p style="text-align:justify;">
 
-
Insert text here
 
-
</p>
 
</html>
</html>
{{end}}
{{end}}

Latest revision as of 13:14, 19 September 2011

UNIPV TEAM 2011


The project

Our work aims at implementing the engineering concept of closed-loop control in E. coli, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.


You can add captions too…



Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia"