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| - | Abstract
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| - | <div style= "text-align:center "><div class="thumbinner" style="width: 800px;"><img src="https://static.igem.org/mediawiki/2011/b/b1/UNIPV_Pavia_Work_in_progress_scribblings.jpg" class="thumbimage" width=" 89%"></a></div></div> | + | <tr> |
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| | + | <img alt="" src="https://static.igem.org/mediawiki/2011/b/b1/UNIPV_Pavia_Work_in_progress_scribblings.jpg" class="thumbimage" width="93%"></a></div></div> |
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| | + | <h2 class="art-postheader">The project</h2> |
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| | <p align='justify'>Our work aims at implementing the engineering concept of closed-loop control in <em>E. coli</em>, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. | | <p align='justify'>Our work aims at implementing the engineering concept of closed-loop control in <em>E. coli</em>, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. |
| | To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach. | | To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach. |
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| - | <h2 class="art-postheader">Gallery</h2>
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| | <div class="slider-wrapper theme-default"> | | <div class="slider-wrapper theme-default"> |
| | <div class="ribbon"></div> | | <div class="ribbon"></div> |
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| - | <div id="nivoslider-125" class="nivoSlider" style="width:700px;height:470px;"> | + | <div id="nivoslider-125" class="nivoSlider" style="width:550px;height:366px;"> |
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| - | <img src="https://static.igem.org/mediawiki/2011/8/81/UNIPVteam1.jpg" title="UNIPV iGEM team" alt="" />
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| - | <img src="https://static.igem.org/mediawiki/2011/f/fd/UNIPVteam2.jpg" title="UNIPV iGEM team" alt="" />
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| - | <img src="https://static.igem.org/mediawiki/2011/1/13/UNIPV_team3.jpg" title="UNIPV iGEM team" alt="" />
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| - | <img src="https://static.igem.org/mediawiki/2011/d/d3/UNIPV_Team4.jpg" title="UNIPV iGEM team" alt="" />
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| | + | <img src="https://static.igem.org/mediawiki/2011/2/2d/UNIPV_Hometeam1.jpg" title="UNIPV iGEM team" alt="" /> |
| | + | <img src="https://static.igem.org/mediawiki/2011/0/0a/UNIPV_Hometeam2.jpg" title="UNIPV iGEM team" alt="" /> |
| | + | <img src="https://static.igem.org/mediawiki/2011/e/e1/UNIPV_Hometeam3.jpg" title="UNIPV iGEM team" alt="" /> |
| | + | <img src="https://static.igem.org/mediawiki/2011/f/f2/UNIPV_Hometeam4.jpg" title="Work in progress" alt="" /> |
| | + | <img src="https://static.igem.org/mediawiki/2011/9/9b/UNIPV_Home1.jpg" title="Pavia and the river Ticino" alt="" /> |
| | + | <img src="https://static.igem.org/mediawiki/2011/a/a2/UNIPV_Home2.jpg" title="University of Pavia" alt="" /> |
| | + | <img src="https://static.igem.org/mediawiki/2011/f/f7/UNIPV_Home3.jpg" title="Faculty of Engineering" alt="" /> |
| | + | <img src="https://static.igem.org/mediawiki/2011/f/f0/UNIPV_Home4.jpg" title="Scientific campus" alt="" /> |
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| | </div> | | </div> |
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