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SEPTEMBER: WEEK 1
September, 2ndE3-1C3-2 was purified in order to screen the part length.
R0040 in J61002-1C3, E3N-1C3 and J101-31N-1C3 were transformed in 200 μl of TOP10 competent cells.
September, 3rd
All the cultures were diluted 1:200 in M9 with the proper antibiotic. After two hours T9002-ENTERO and ENTERO-RBS were aliquoted in 96-well microplate and induced with known concentrations of 3OC6-HSL and with the supernatants collected on August, 31st.
 Small size gel
The sample did not show the correct band.
September, 4thR0040 in J61002-1C3-1, R0040 in J61002-1C3-2, J101-31-1C3-1 and J101-31-1C3-2 were glycerol stocked and then plasmidic DNA was purified with MiniPrep commercial kit:
2.5 μl of each sample were digested with 0.5 μl EcoRI and PstI resctriction endonucleases in a 25 μl final volume. A small size agarose gel was prepared. In the afternoon electrophoresis was carried out; all samples showed the correct insert.  Small size gel
Inoculum of T9002-ENTERO and ENTERO-RBS to measure 3OC6-HSL concentration in supernatants collected on August, 31st.
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Team:UNIPV-Pavia/Calendar/September/week1
From 2011.igem.org
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Latest revision as of 10:16, 18 September 2011
