Team:UNICAMP-EMSE Brazil/Notebook/17 September 2011

From 2011.igem.org

(Difference between revisions)
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==Building our devices==
==Building our devices==
 +
[[Image:UNICAMP-EMSE Brazil Gel4 17 09 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 4, 17/09/2011.'''</font> <br>Gel 4: Ladder / pConst+RBS+SoxR+T C1 (D)/ pConst+RBS+SoxR+T C2 (D)/ pConst+RBS+SoxR+T C3 (D)/ pConst+RBS+SoxR+T C4 (D)/ pConst+RBS+SoxR+T (ND) / SoxS+RBS+IL-10+HlyA+T C1 (D)/ SoxS+RBS+IL-10+HlyA+T C2 (D)/ SoxS+RBS+IL-10+HlyA+T C3 (D)/ SoxS+RBS+IL-10+HlyA+T C4 (D)/ SoxS+RBS+IL-10+HlyA+T (ND)/ flhDC+RBS+IL-12+HlyA+T (D) / flhDC+RBS+IL-12+HlyA+T (ND)/ flhDC+RBS+GFP+HlyA+T  (D)/ flhDC+RBS+GFP+HlyA+T (ND)/ SoxS+RBS+GFP+HlyA+T  (D)/ SoxS+RBS+GFP+HlyA+T Test  (D)/ SoxS+RBS+GFP+HlyA+T  (ND) / pConst+RBS+QseB+RBS+QseC+T  (D)/ pConst+RBS+QseB+RBS+QseC+T  (ND) ]]
 +
[[Image:UNICAMP-EMSE Brazil Gel5 17 09 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 5, 17/09/2011.'''</font> <br>Gel 5: Ladder / flhDC+RBS+HlyB+RBS+HlyD+TolC+T (D)/ flhDC+RBS+HlyB+RBS+HlyD+TolC+T (ND)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (D)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (ND)/ pSB1C3 TolC C10 (D) / pSB1C3 TolC C10 (ND) ]]
 +
==='''Quantification and checking digestion of sequences to ship'''===
==='''Quantification and checking digestion of sequences to ship'''===
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'''Samples:'''   
'''Samples:'''   
:*'''Gel 1:'''
:*'''Gel 1:'''
-
{|border="1" cellpadding="5" cellspacing="0" align="center"
+
::*Ladder / pConst+RBS+SoxR+T C1 (D)/ pConst+RBS+SoxR+T C2 (D)/ pConst+RBS+SoxR+T C3 (D)/ pConst+RBS+SoxR+T C4 (D)/ pConst+RBS+SoxR+T (ND) / SoxS+RBS+IL-10+HlyA+T C1 (D)/ SoxS+RBS+IL-10+HlyA+T C2 (D)/ SoxS+RBS+IL-10+HlyA+T C3 (D)/ SoxS+RBS+IL-10+HlyA+T C4 (D)/ SoxS+RBS+IL-10+HlyA+T (ND)/ flhDC+RBS+IL-12+HlyA+T (D) / flhDC+RBS+IL-12+HlyA+T (ND)/ flhDC+RBS+GFP+HlyA+T  (D)/ flhDC+RBS+GFP+HlyA+T (ND)/ SoxS+RBS+GFP+HlyA+T  (D)/ SoxS+RBS+GFP+HlyA+T Test (D)/ SoxS+RBS+GFP+HlyA+T  (ND) / pConst+RBS+QseB+RBS+QseC+T  (D)/ pConst+RBS+QseB+RBS+QseC+T  (ND)
-
|-
+
-
! scope="col" style="background:#efefef;" | Ladder
+
-
! scope="col" style="background:#efefef;" | pConst+RBS+SoxR+T C1 (D)
+
-
! scope="col" style="background:#efefef;" | pConst+RBS+SoxR+T C2 (D)
+
-
! scope="col" style="background:#efefef;" | pConst+RBS+SoxR+T C3 (D)
+
-
! scope="col" style="background:#efefef;" | pConst+RBS+SoxR+T C4 (D)
+
-
! scope="col" style="background:#efefef;" | pConst+RBS+SoxR+T (ND)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+IL-10+HlyA+T C1 (D)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+IL-10+HlyA+T C2 (D)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+IL-10+HlyA+T C3 (D)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+IL-10+HlyA+T C4 (D)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+IL-10+HlyA+T (ND)
+
-
! scope="col" style="background:#efefef;" | flhDC+RBS+IL-12+HlyA+T (D)
+
-
! scope="col" style="background:#efefef;" | flhDC+RBS+IL-12+HlyA+T (ND)
+
-
! scope="col" style="background:#efefef;" | flhDC+RBS+GFP+HlyA+T  (D)
+
-
! scope="col" style="background:#efefef;" | flhDC+RBS+GFP+HlyA+T (ND)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+GFP+HlyA+T  (D)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+GFP+HlyA+T Test (D)
+
-
! scope="col" style="background:#efefef;" | SoxS+RBS+GFP+HlyA+T  (ND)
+
-
! scope="col" style="background:#efefef;" | pConst+RBS+QseB+RBS+QseC+T  (D)
+
-
! scope="col" style="background:#efefef;" | pConst+RBS+QseB+RBS+QseC+T  (ND)
+
-
|}</font>
+
:*'''Gel 2:'''
:*'''Gel 2:'''
-
::*Ladder / pConst+RBS+SoxR+T C1 (D)/ pConst+RBS+SoxR+T C2 (D)/ pConst+RBS+SoxR+T C3 (D)/ pConst+RBS+SoxR+T C4 (D)/ pConst+RBS+SoxR+T (ND) / SoxS+RBS+IL-10+HlyA+T C1 (D)/ SoxS+RBS+IL-10+HlyA+T C2 (D)/ SoxS+RBS+IL-10+HlyA+T C3 (D)/ SoxS+RBS+IL-10+HlyA+T C4 (D)/ SoxS+RBS+IL-10+HlyA+T (ND)/ flhDC+RBS+IL-12+HlyA+T (D) / flhDC+RBS+IL-12+HlyA+T (ND)/ flhDC+RBS+GFP+HlyA+T  (D)/ flhDC+RBS+GFP+HlyA+T (ND)/ SoxS+RBS+GFP+HlyA+T  (D)/ SoxS+RBS+GFP+HlyA+T Test  (D)/ SoxS+RBS+GFP+HlyA+T (ND) / pConst+RBS+QseB+RBS+QseC+T  (D)/ pConst+RBS+QseB+RBS+QseC+T  (ND)
+
::*Ladder / flhDC+RBS+HlyB+RBS+HlyD+TolC+T (D)/ flhDC+RBS+HlyB+RBS+HlyD+TolC+T (ND)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (D)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (ND)/ pSB1C3 TolC C10 (D) / pSB1C3 TolC C10 (ND)
*'''Ladder:'''  
*'''Ladder:'''  

Revision as of 01:27, 18 September 2011

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Contents

Notebook

Click on a date to see what we have done!

May
MTWTFSS
            [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_May_2011&action=edit 1]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_May_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_May_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_May_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_May_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_May_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_May_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_May_2011&action=edit 8]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_May_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_May_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_May_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_May_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_May_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_May_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_May_2011&action=edit 15]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_May_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_May_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_May_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_May_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_May_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_May_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_May_2011&action=edit 22]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_May_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_May_2011&action=edit 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_May_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_May_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_May_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_May_2011&action=edit 29]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_May_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_May_2011 31]
June
MTWTFSS
    [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_June_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_June_2011 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_June_2011&action=edit 5]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_June_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_June_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_June_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_June_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_June_2011&action=edit 12]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_June_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_June_2011&action=edit 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_June_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_June_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_June_2011&action=edit 19]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_June_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_June_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_June_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_June_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_June_2011&action=edit 26]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_June_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_June_2011&action=edit 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_June_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_June_2011&action=edit 30]
July
MTWTFSS
        [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_July_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_July_2011&action=edit 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_July_2011 3]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_July_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_July_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_July_2011&action=edit 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_July_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_July_2011&action=edit 10]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_July_2011 11] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_July_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_July_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_July_2011 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_July_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_July_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_July_2011&action=edit 17]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/18_July_2011 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_July_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_July_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_July_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_July_2011&action=edit 24]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_July_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_July_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_July_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_July_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_August_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_August_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_August_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_August_2011 19] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_August_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_August_2011 21]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_August_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_August_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_August_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_August_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_August_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_August_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_August_2011&action=edit 28]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_August_2011 29] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_August_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_August_2011 31]
September
MTWTFSS
      [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_September_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_September_2011&action=edit 4]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/5_September_2011 5] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_September_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_September_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_September_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_September_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_September_2011 10] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_September_2011 11]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_September_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_September_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_September_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_September_2011&action=edit 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_September_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_September_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_September_2011&action=edit 18]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_September_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_September_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_September_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_September_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_September_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_September_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_September_2011 25]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_September_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_September_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_September_2011 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_September_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_October_2011&action=edit 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_October_2011 2]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_October_2011&action=edit 31]


Electrophoresis Gel 1, 17/09/2011.
Gel 1: pSB1/ pSB 2/ pSB 3/ pSB 4/ pSB 5/ RBS+IL-12 C4/ RBS+IL-12 C5/ RBS+HlyD C4/ RBS+HlyD C5/ RBS+Tol C C10/ RBS+Tol C C11/ RBS+QseB+RBS+QseC C10/ RBS+QseB+RBS+QseC C11/ RBS+QseB+RBS+QseC C12/
Electrophoresis Gel 2, 17/09/2011.
RBS+SoxS C10/ RBS+SoxS C11/ RBS+SoxS C12/ RBS+SoxS C13/ RBS+HlyB C10/ RBS+HlyB C11/ RBS+HlyB C12/ SoxS+ RBS+HlyB +RBS+HlyD+ RBS+TolC+T C1 1/ SoxS+ RBS+HlyB+ RBS+HlyD + RBS+TolC+T C1 2/ SoxS+ RBS+HlyB+ RBS+HlyD + RBS+TolC+T C3/

17 September 2011

Sequences to ship

Quantification and checking digestion of sequences to ship

Objectives:

  1. Quantification of linearized pSB1C3 vector purified from the gel (volume of ladder applied 6 uL; sample: 5 uL DNA +1 uL buffer),
  2. Checking digestion ( EcoRI and PstI) of samples mini-preped 09/15 and 09/16.
  3. Checking digestion of SoxS+HlyB+HlyD +TolC+T C1 1/ SoxS+HlyB+HlyD +TolC+T C1 2/ SoxS+HlyB+HlyD +TolC+T C1 3 (EcoRI and PstI) of samples mini-preped 09/08.

Samples:

  • Gel 1:
  • pSB1/ pSB 2/ pSB 3/ pSB 4/ pSB 5/ RBS+IL-12 C4/ RBS+IL-12 C5/ RBS+HlyD C4/ RBS+HlyD C5/ RBS+Tol C C10/ RBS+Tol C C11/ RBS+QseB+RBS+QseC C10/ RBS+QseB+RBS+QseC C11/ RBS+QseB+RBS+QseC C12/
  • Gel 2:
  • RBS+SoxS C10/ RBS+SoxS C11/ RBS+SoxS C12/ RBS+SoxS C13/ RBS+HlyB C10/ RBS+HlyB C11/ RBS+HlyB C12/ SoxS+ RBS+HlyB +RBS+HlyD+ RBS+TolC+T C1 1/ SoxS+ RBS+HlyB+ RBS+HlyD + RBS+TolC+T C1 2/ SoxS+ RBS+HlyB+ RBS+HlyD + RBS+TolC+T C3/
  • Ladder:
  • 100-10.000 bp (Fermentas)
  • Gel Agarose concentration:
  • 1,0%


  • Results:

UNICAMP-EMSE Brazil Table 17 09.jpg



Electrophoresis Gel 3, 17/09/2011.
Gel 3: (d)RBS+HlyD C4/ (nd)RBS+HlyD C4/ (d)RBS+Tol C C11/ (nd)RBS+Tol C C11/ (d)RBS+QseB+RBS+QseC C10/ (nd)RBS+QseB+RBS+QseC C10/(d)RBS+SoxS C13/ (nd)RBS+SoxS C13/ (d)RBS+HlyB C10/ (nd)RBS+HlyB C10/ (d)RBS+HlyB C11/(nd) RBS+HlyB C11/ (d)SoxS+ RBS+HlyB +RBS+HlyD+ RBS+TolC+T C1 1/(nd) SoxS+ RBS+HlyB +RBS+HlyD+ RBS+TolC+T C1 1

Revising constructs

Objective:

  1. Checking digestion with Eco RI of some constructs that had strange result in former electrophoresis

Samples:

  • Gel 3:
  • (d)RBS+HlyD C4/ (nd)RBS+HlyD C4/ (d)RBS+Tol C C11/ (nd)RBS+Tol C C11/ (d)RBS+QseB+RBS+QseC C10/ (nd)RBS+QseB+RBS+QseC C10/(d)RBS+SoxS C13/ (nd)RBS+SoxS C13/ (d)RBS+HlyB C10/ (nd)RBS+HlyB C10/ (d)RBS+HlyB C11/(nd) RBS+HlyB C11/ (d)SoxS+ RBS+HlyB +RBS+HlyD+ RBS+TolC+T C1 1/(nd) SoxS+ RBS+HlyB +RBS+HlyD+ RBS+TolC+T C1 1


  • Results:

UNICAMP-EMSE Brazil Table2 17 09.jpg


Building our devices

Electrophoresis Gel 4, 17/09/2011.
Gel 4: Ladder / pConst+RBS+SoxR+T C1 (D)/ pConst+RBS+SoxR+T C2 (D)/ pConst+RBS+SoxR+T C3 (D)/ pConst+RBS+SoxR+T C4 (D)/ pConst+RBS+SoxR+T (ND) / SoxS+RBS+IL-10+HlyA+T C1 (D)/ SoxS+RBS+IL-10+HlyA+T C2 (D)/ SoxS+RBS+IL-10+HlyA+T C3 (D)/ SoxS+RBS+IL-10+HlyA+T C4 (D)/ SoxS+RBS+IL-10+HlyA+T (ND)/ flhDC+RBS+IL-12+HlyA+T (D) / flhDC+RBS+IL-12+HlyA+T (ND)/ flhDC+RBS+GFP+HlyA+T (D)/ flhDC+RBS+GFP+HlyA+T (ND)/ SoxS+RBS+GFP+HlyA+T (D)/ SoxS+RBS+GFP+HlyA+T Test (D)/ SoxS+RBS+GFP+HlyA+T (ND) / pConst+RBS+QseB+RBS+QseC+T (D)/ pConst+RBS+QseB+RBS+QseC+T (ND)
Electrophoresis Gel 5, 17/09/2011.
Gel 5: Ladder / flhDC+RBS+HlyB+RBS+HlyD+TolC+T (D)/ flhDC+RBS+HlyB+RBS+HlyD+TolC+T (ND)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (D)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (ND)/ pSB1C3 TolC C10 (D) / pSB1C3 TolC C10 (ND)


Quantification and checking digestion of sequences to ship

Objectives:

  1. Finishing the assembly of our devices
  1. Secretion device (containing HlyB, HlyD and TolC in two manners: regulated by flhDC promoter or SoxS promoter)
  2. Th1-directed device (containing QseC and QseB sensor proteins constitutively expressed and IL-12 whose expression is regulated by flhDC promoter)
  3. Th2-directed device (containing the SoxR sensor protein constitutively expressed and IL-10 whose expression is regulated by SoxS promoter)
  1. After finishing the assembly of these parts, we aim to put together the plasmid containing the Secretion system (1.1) and the plasmid containing the sensor-response systems (the one that senses Adrenaline and responds by producing IL-12 (1.2) and the one which senses Nitric Oxide and responds by producing IL-10 (1.3).

Samples:

  • Gel 1:
  • Ladder / pConst+RBS+SoxR+T C1 (D)/ pConst+RBS+SoxR+T C2 (D)/ pConst+RBS+SoxR+T C3 (D)/ pConst+RBS+SoxR+T C4 (D)/ pConst+RBS+SoxR+T (ND) / SoxS+RBS+IL-10+HlyA+T C1 (D)/ SoxS+RBS+IL-10+HlyA+T C2 (D)/ SoxS+RBS+IL-10+HlyA+T C3 (D)/ SoxS+RBS+IL-10+HlyA+T C4 (D)/ SoxS+RBS+IL-10+HlyA+T (ND)/ flhDC+RBS+IL-12+HlyA+T (D) / flhDC+RBS+IL-12+HlyA+T (ND)/ flhDC+RBS+GFP+HlyA+T (D)/ flhDC+RBS+GFP+HlyA+T (ND)/ SoxS+RBS+GFP+HlyA+T (D)/ SoxS+RBS+GFP+HlyA+T Test (D)/ SoxS+RBS+GFP+HlyA+T (ND) / pConst+RBS+QseB+RBS+QseC+T (D)/ pConst+RBS+QseB+RBS+QseC+T (ND)
  • Gel 2:
  • Ladder / flhDC+RBS+HlyB+RBS+HlyD+TolC+T (D)/ flhDC+RBS+HlyB+RBS+HlyD+TolC+T (ND)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (D)/ SoxS+RBS+ HlyB+RBS+HlyD+TolC+T (ND)/ pSB1C3 TolC C10 (D) / pSB1C3 TolC C10 (ND)
  • Ladder:
  • 100-10.000 bp (Fermentas)
  • Gel Agarose concentration:
  • 1,0%


  • Results:




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