Team:UNIPV-Pavia/Tecan Measurements

From 2011.igem.org

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<li><b>04-08-2011</b> preliminary test on PtetR (E32, E33, E34, E35 induced with 0, 1, 10, 50, 100 ng/ml atc)
-
 
+
<li><b>05-08-2011</b> preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 3rd
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+
<li><b>17-08-2011</b> preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc, diluted to 1:200 and 1:500 in microplate) SN collected on August, 3rd
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+
<li><b>18-08-2011</b> PtetR with RBS30 and RBS31 (E32, E33 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
 +
<li><b>20-08-2011</b> PtetR with RBS32 and RBS34 (E34, E35 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
 +
<li><b>23-08-2011</b> the day TECAN broke down! (We should have performed a test on SN of AiiA LC E37, E38, E39, E40 induced with 75 ng/ml atc)
 +
<li><b>24-08-2011</b> Plux with RBS30 and RBS31 (E17, E18 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
 +
<li><b>25-08-2011</b> Plux with RBS32 and RBS34 (E19, E20 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
 +
<li><b>26-08-2011</b> preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 19th
 +
<li><b>29-08-2011</b> preliminary test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on August, 27th
 +
<li><b>03-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
 +
<li><b>05-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
 +
<li><b>05-09-2011</b> test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd (0 h, 7 h, 24 h)
 +
<li><b>06-09-2011</b> test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th (0 h, 2h, 6 h)
 +
<li><b>07-09-2011</b> test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th (0 h, 5 h, 24 h)
 +
<li><b>08-09-2011</b> test on T9002 sensitivity
 +
<li><b>09-09-2011</b> test on AiiA HC in MGZ1 (E24, E25, E26, E27 induced with 100 ng/ml atc) SN collected on Septmeber, 8th (0 h, 1 h, 2 h, 4 h, 7 h)
 +
<li><b>10-09-2011</b> test on SN at different pHs (5.9, 6.3, 6.7, 6.9 and 7.2 collected on September, 8th from ENTERO-4C5 cultures and M9 medium without cells)
 +
<li><b>10-09-2011</b> test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 10th (0 h, 1 h)
 +
<li><b>11-09-2011</b> test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 10th (2 h, 4 h)
 +
<li><b>13-09-2011</b> test on luxI producing HSL (E31, E41, E42, E43 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 12th (0 h, 1 h)
 +
<li><b>13-09-2011</b> test on luxI producing HSL (E31, E41, E42, E43 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 12th (2 h, 4 h)
 +
<li><b>15-09-2011</b> test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 13th (0 h, 1 h)
 +
<li><b>15-09-2011</b> test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 13th (2 h, 4 h)
 +
<li><b>16-09-2011</b> test on SN of negative non-degrading cultures collected on Septmeber, 14th (2 h, 4 h)
 +
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Latest revision as of 19:18, 16 September 2011

UNIPV TEAM 2011

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TECAN MEASUREMENTS

  • 04-08-2011 preliminary test on PtetR (E32, E33, E34, E35 induced with 0, 1, 10, 50, 100 ng/ml atc)
  • 05-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 3rd
  • 17-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc, diluted to 1:200 and 1:500 in microplate) SN collected on August, 3rd
  • 18-08-2011 PtetR with RBS30 and RBS31 (E32, E33 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
  • 20-08-2011 PtetR with RBS32 and RBS34 (E34, E35 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
  • 23-08-2011 the day TECAN broke down! (We should have performed a test on SN of AiiA LC E37, E38, E39, E40 induced with 75 ng/ml atc)
  • 24-08-2011 Plux with RBS30 and RBS31 (E17, E18 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
  • 25-08-2011 Plux with RBS32 and RBS34 (E19, E20 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
  • 26-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 19th
  • 29-08-2011 preliminary test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on August, 27th
  • 03-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
  • 05-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
  • 05-09-2011 test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd (0 h, 7 h, 24 h)
  • 06-09-2011 test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th (0 h, 2h, 6 h)
  • 07-09-2011 test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th (0 h, 5 h, 24 h)
  • 08-09-2011 test on T9002 sensitivity
  • 09-09-2011 test on AiiA HC in MGZ1 (E24, E25, E26, E27 induced with 100 ng/ml atc) SN collected on Septmeber, 8th (0 h, 1 h, 2 h, 4 h, 7 h)
  • 10-09-2011 test on SN at different pHs (5.9, 6.3, 6.7, 6.9 and 7.2 collected on September, 8th from ENTERO-4C5 cultures and M9 medium without cells)
  • 10-09-2011 test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 10th (0 h, 1 h)
  • 11-09-2011 test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 10th (2 h, 4 h)
  • 13-09-2011 test on luxI producing HSL (E31, E41, E42, E43 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 12th (0 h, 1 h)
  • 13-09-2011 test on luxI producing HSL (E31, E41, E42, E43 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 12th (2 h, 4 h)
  • 15-09-2011 test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 13th (0 h, 1 h)
  • 15-09-2011 test on AiiA LC in MGZ1 (E37, E38, E39, E40 induced with 6 ng/ml, 8 ng/ml and 100 ng/ml atc) SN collected on Septmeber, 13th (2 h, 4 h)
  • 16-09-2011 test on SN of negative non-degrading cultures collected on Septmeber, 14th (2 h, 4 h)

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