Team:Washington/Protocols

From 2011.igem.org

(Difference between revisions)
m
Line 47: Line 47:
=Make It: Magnetosome Protocols=
=Make It: Magnetosome Protocols=
-
[https://2011.igem.org/Team:Washington/Protocols/Gib_Rxn Gibson Reaction]
+
[https://2011.igem.org/Team:Washington/Protocols/Gib_Rxn Gibson Cloning/Assembly]
[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification]
[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification]
-
=Gibson Vectors (pGB) protocols=
+
 

Revision as of 04:00, 14 September 2011


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagensis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks


Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction


Break It: Gluten Destruction Protocols

Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification


Make It: Magnetosome Protocols

Gibson Cloning/Assembly

Gibson Purification




Wiki Design

Wiki Design Tools

Retrieved from "http://2011.igem.org/Team:Washington/Protocols"