Team:Washington/Protocols
From 2011.igem.org
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[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification] | [https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification] | ||
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=Gibson Vectors (pGB) protocols= | =Gibson Vectors (pGB) protocols= |
Revision as of 22:43, 13 September 2011
General Protocols
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: Magnetosome Protocols