Team:UNIPV-Pavia/Tecan Measurements

From 2011.igem.org

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<li><b>03-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
<li><b>03-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
<li><b>05-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
<li><b>05-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
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<li><b>05-09-2011</b> test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd
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<li><b>05-09-2011</b> test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd (0 h, 7 h, 24 h)
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<li><b>06-09-2011</b> test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th
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<li><b>06-09-2011</b> test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th (0 h, 2h, 6 h)
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<li><b>07-09-2011</b> test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th)
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<li><b>07-09-2011</b> test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th (0 h, 5 h, 24 h)
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Revision as of 21:42, 7 September 2011

UNIPV TEAM 2011

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August
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September
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October
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TECAN MEASUREMENTS

  • 04-08-2011 preliminary test on PtetR (E32, E33, E34, E35 induced with 0, 1, 10, 50, 100 ng/ml atc)
  • 05-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 3rd
  • 17-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc, diluted to 1:200 and 1:500 in microplate) SN collected on August, 3rd
  • 18-08-2011 PtetR with RBS30 and RBS31 (E32, E33 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
  • 20-08-2011 PtetR with RBS32 and RBS34 (E34, E35 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
  • 23-08-2011 the day TECAN broke down! (We should have performed a test on SN of AiiA LC E37, E38, E39, E40 induced with 75 ng/ml atc)
  • 24-08-2011 Plux with RBS30 and RBS31 (E17, E18 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
  • 25-08-2011 Plux with RBS32 and RBS34 (E19, E20 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
  • 26-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 19th
  • 29-08-2011 preliminary test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on August, 27th
  • 03-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
  • 05-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
  • 05-09-2011 test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd (0 h, 7 h, 24 h)
  • 06-09-2011 test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th (0 h, 2h, 6 h)
  • 07-09-2011 test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th (0 h, 5 h, 24 h)

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