Team:Freiburg/Notebook/5 September

From 2011.igem.org

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Minipreps of the first inoculations were applied on a 1% agarose gel without digesting them to see if they have an insert of the modified Lysis genes K124017. Nr1, 5 and 7 were sent for sequencing since the size seems to be about right.
Minipreps of the first inoculations were applied on a 1% agarose gel without digesting them to see if they have an insert of the modified Lysis genes K124017. Nr1, 5 and 7 were sent for sequencing since the size seems to be about right.
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*Note: S4 is the B0034 RBS (ribosome binding site), S15 is the non-modified K124017 Lysis Cassette without RBS
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Revision as of 18:04, 6 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

green light receptor

transformation with reporter parts

Investigators:Julia

transformation with iGEM parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15016 BBa_I15016] which is B0032.ECFP
and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15017 BBa_I15017 ]which is B0032.EYFP.
Tomorrow the green light promotor from PCR of 29_August should be inserted infront of the reporter proteins CFP and YFP.

blue light receptor

Miniprep

Investigators:Sophie
continue from: picking clones; 04.09.11

Testdigest showed no inserts

Ligation

Investigators: Sophie
again ligation as last time (02.08.11)

Lysis cassette

3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017

Investigators:Theo


Minipreps of the first inoculations were applied on a 1% agarose gel without digesting them to see if they have an insert of the modified Lysis genes K124017. Nr1, 5 and 7 were sent for sequencing since the size seems to be about right.

  • Note: S4 is the B0034 RBS (ribosome binding site), S15 is the non-modified K124017 Lysis Cassette without RBS


Freiburg11 9 7 2011 Gel von S4plusS15 ohne Verdau.Jpg


Precipitator

Miniprep

Investigators: Sophie
continue from experiment: Transformation; 03.09.11
testdigest showed no inserts

Trafo

Investigators. Sandra

Repeat of the trafo of:

  1. pGEX vector (Amp. resistance)
  2. pGEX vector (Amp. resistance)
  3. pGEX vector (Amp. resistance)
  4. pSB1C3
  5. pET Duet vector (Amp. resistance)
  6. pET Duet vector (Amp. resistance)
  7. pET Duet vector (Amp. resistance)
  8. pSB1C3
  9. pSB1C3
  10. pSB1C3

                       

Trafo

Investigators. Sandra

Repeat of the trafo of:

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