Team:Freiburg/Notebook/5 September

• < home >
• < team >
  • → members
  • → attributions
  • → freiburg
• < project >
  • → description
  • → results
  • → modeling
  • → notebook
  • → sample data
• < human practices >
  • → oath
  • → ethics
  • → safety
• < gallery >
• < sponsors >
• < to the forums >

green light receptor

transformation with reporter parts

Investigators:Julia

transformation with iGEM parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15016 BBa_I15016] which is B0032.ECFP
and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15017 BBa_I15017 ]which is B0032.EYFP.
Tomorrow the green light promotor from PCR of 29_August should be inserted infront of the reporter proteins CFP and YFP.

blue light receptor

Miniprep

Investigators:Sophie
continue from: picking clones; 04.09.11

Testdigest showed no inserts

Ligation

Investigators: Sophie
again ligation as last time (02.08.11)

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

Miniprep

Investigators: Sophie
continue from experiment: Transformation; 03.09.11
testdigest showed no inserts

Trafo

Investigators. Sandra

Repeat of the trafo of:

1. pGEX vector (Amp. resistance)
2. pGEX vector (Amp. resistance)
3. pGEX vector (Amp. resistance)
4. pSB1C3
5. pET Duet vector (Amp. resistance)
6. pET Duet vector (Amp. resistance)
7. pET Duet vector (Amp. resistance)
8. pSB1C3
9. pSB1C3
10. pSB1C3