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Team:Paris Liliane Bettencourt/Notebook/2011/08/31/
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The sequencing results arrived and it appeared that only the cloning of the promoter Hyperspank in pSB1C3 succeeded. | The sequencing results arrived and it appeared that only the cloning of the promoter Hyperspank in pSB1C3 succeeded. | ||
I then launched the clons in liquid culture for glycerols it would be the strain S87. | I then launched the clons in liquid culture for glycerols it would be the strain S87. | ||
| + | |||
| + | ==Mathias & Laura== | ||
| + | |||
| + | We tried to insert the Biobricks "LacI controled T7 and reporter for Bacillus" and "T7 Autoamplifier system + Reporter" into pHM3 | ||
| + | |||
| + | ===Miniprep=== | ||
| + | |||
| + | -LacI controled T7 and reporter for Bacillus | ||
| + | |||
| + | -T7 Autoamplifier system + Reporter | ||
| + | |||
| + | -pHM3 | ||
| + | |||
| + | ===Digestion and gel extraction=== | ||
| + | |||
| + | -LacI controled T7 and reporter for Bacillus by EcoR1 and Pst1 | ||
| + | |||
| + | -T7 Autoamplifier system + Reporter by EcoR1 and Pst1 | ||
| + | |||
| + | -pHM3 by EcoR1 and Pst1 | ||
| + | |||
| + | ===Ligation and transformation=== | ||
| + | |||
| + | -LacI controled T7 and reporter for Bacillus & pHM3 | ||
| + | |||
| + | -T7 Autoamplifier system + Reporter & pHM3 | ||
Revision as of 16:33, 3 September 2011
Contents |
Axel
extraction of genomic DNA (CodY-)
TECAN quantification of DNA: OD260=47,9ng/µL
Camille
The sequencing results arrived and it appeared that only the cloning of the promoter Hyperspank in pSB1C3 succeeded. I then launched the clons in liquid culture for glycerols it would be the strain S87.
Mathias & Laura
We tried to insert the Biobricks "LacI controled T7 and reporter for Bacillus" and "T7 Autoamplifier system + Reporter" into pHM3
Miniprep
-LacI controled T7 and reporter for Bacillus
-T7 Autoamplifier system + Reporter
-pHM3
Digestion and gel extraction
-LacI controled T7 and reporter for Bacillus by EcoR1 and Pst1
-T7 Autoamplifier system + Reporter by EcoR1 and Pst1
-pHM3 by EcoR1 and Pst1
Ligation and transformation
-LacI controled T7 and reporter for Bacillus & pHM3
-T7 Autoamplifier system + Reporter & pHM3
